Journal article
EWS-ATF-1 chimeric protein in soft tissue clear cell sarcoma associates with CREB-binding protein and interferes with p53-mediated trans-activation function
Oncogene, v 20(46), pp 6653-6659
01 Oct 2001
PMID: 11709699
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The recurrent t(12; 22) (q13; q12) chromosomal translocation associated with soft tissue clear cell sarcoma results in a chimeric protein EWS-ATF-1 that acts as a constitutive transcriptional activator. The CBP/p300 transcriptional coactivator, which links various transcriptional factors to basal transcription apparatus, participates in transcriptional activation, growth and cell cycle control and differentiation. In this study, we show that EWS-ATF-1 associates constitutively with CBP both in vitro and in vivo. Both EWS and ATF-1 fusion domains are needed for this interaction. Here, we demonstrate that EWS-ATF-1 represses p53/CBP-mediated trans-activation function. Overexpression of CBP can counteract this repressive effect of EWS-ATF-1. Taken together, these findings suggest that one of the mechanisms by which EWS-ATF-1 may cause tumors is through targeting CBP/p300 resulting in the loss of function of p53. This novel mechanism may be responsible for the development of these and other related solid tumors.
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Details
- Title
- EWS-ATF-1 chimeric protein in soft tissue clear cell sarcoma associates with CREB-binding protein and interferes with p53-mediated trans-activation function
- Creators
- Yasuo Fujimura - Hahnemann University HospitalH Siddique - Hahnemann University HospitalLeo Lee - Science Applications International CorporationV Rao - Hahnemann University HospitalESP ReddyLinda Y Lee - College of Engineering (1970-)
- Publication Details
- Oncogene, v 20(46), pp 6653-6659
- Publisher
- Springer Nature
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Engineering
- Web of Science ID
- WOS:000171551600001
- Scopus ID
- 2-s2.0-0035845854
- Other Identifier
- 991019168138004721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology
- Genetics & Heredity
- Oncology