Journal article
Early detection of de novo hepatitis C infection in patients after liver transplantation by reverse transcriptase polymerase chain reaction
Surgery, v 114(2), pp 442-448
01 Aug 1993
PMID: 7688155
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
BACKGROUNDReverse transcriptase polymerase chain reaction (RT-PCR) can detect the viral genome and show hepatitis C recurrence in patients who undergo transplantation for chronic hepatitis C viral (HCV) infection. We investigated the utility of an RT-PCR-based HCV assay for early detection of viral RNA in de novo HCV infection after liver transplantation.METHODSPretransplantation antibodies and explanation HCV viral RNA status were obtained from 117 patients. Follow-up liver biopsy specimens were examined for evidence of hepatitis activity. Plasma samples during the period of time of the biopsy were assayed for HCV antibody and viral RNA. RNA was extracted from samples and reverse transcribed to cDNA. cDNA was amplified by PCR, and products were detected by liquid hybridization.RESULTSClinical hepatitis developed in seventeen of 117 patients who, before transplantation, were HCV antibody negative and explant viral RNA negative. Ten patients were plasma PCR negative and had known non-hepatitis C causes for the biopsy findings. Of the remaining seven patients, five (70%) were plasma RT-PCR positive before seroconversion in matched plasma samples.CONCLUSIONSIn liver transplant patients, the incidence of de novo clinical hepatitis is low, and HCV viral RNA in de novo clinical hepatitis C infection can be detected in the absence of HCV antibodies.
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Details
- Title
- Early detection of de novo hepatitis C infection in patients after liver transplantation by reverse transcriptase polymerase chain reaction
- Creators
- R MateoA DemetrisE SicoC FryeL F WangY el-SakhawiM ReillyG D EhrlichD CooperJ Fung
- Publication Details
- Surgery, v 114(2), pp 442-448
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:A1993LQ38400040
- Scopus ID
- 2-s2.0-0027225721
- Other Identifier
- 991019183917104721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Surgery