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Journal article
Early inner plexiform layer thinning and retinal nerve fiber layer thickening in excitotoxic retinal injury using deep learning-assisted optical coherence tomography
Acta neuropathologica communications, v 12(1), pp 19-19
01 Feb 2024
PMID: 38303097
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Excitotoxicity from the impairment of glutamate uptake constitutes an important mechanism in neurodegenerative diseases such as Alzheimer's, multiple sclerosis, and Parkinson's disease. Within the eye, excitotoxicity is thought to play a critical role in retinal ganglion cell death in glaucoma, diabetic retinopathy, retinal ischemia, and optic nerve injury, yet how excitotoxic injury impacts different retinal layers is not well understood. Here, we investigated the longitudinal effects of N-methyl-D-aspartate (NMDA)-induced excitotoxic retinal injury in a rat model using deep learning-assisted retinal layer thickness estimation. Before and after unilateral intravitreal NMDA injection in nine adult Long Evans rats, spectral-domain optical coherence tomography (OCT) was used to acquire volumetric retinal images in both eyes over 4 weeks. Ten retinal layers were automatically segmented from the OCT data using our deep learning-based algorithm. Retinal degeneration was evaluated using layer-specific retinal thickness changes at each time point (before, and at 3, 7, and 28 days after NMDA injection). Within the inner retina, our OCT results showed that retinal thinning occurred first in the inner plexiform layer at 3 days after NMDA injection, followed by the inner nuclear layer at 7 days post-injury. In contrast, the retinal nerve fiber layer exhibited an initial thickening 3 days after NMDA injection, followed by normalization and thinning up to 4 weeks post-injury. Our results demonstrated the pathological cascades of NMDA-induced neurotoxicity across different layers of the retina. The early inner plexiform layer thinning suggests early dendritic shrinkage, whereas the initial retinal nerve fiber layer thickening before subsequent normalization and thinning indicates early inflammation before axonal loss and cell death. These findings implicate the inner plexiform layer as an early imaging biomarker of excitotoxic retinal degeneration, whereas caution is warranted when interpreting the ganglion cell complex combining retinal nerve fiber layer, ganglion cell layer, and inner plexiform layer thicknesses in conventional OCT measures. Deep learning-assisted retinal layer segmentation and longitudinal OCT monitoring can help evaluate the different phases of retinal layer damage upon excitotoxicity.
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Details
- Title
- Early inner plexiform layer thinning and retinal nerve fiber layer thickening in excitotoxic retinal injury using deep learning-assisted optical coherence tomography
- Creators
- Da Ma - Wake Forest Univ, Sch Med, 1 Med Ctr Blvd, Winston Salem, NC 27157 USAWenyu Deng - NYU, NYU Langone Hlth, NYU Grossman Sch Med, Dept Ophthalmol, New York, NY 10012 USAZain Khera - NYU, NYU Langone Hlth, NYU Grossman Sch Med, Dept Ophthalmol, New York, NY 10012 USAThajunnisa A. Sajitha - NYU, NYU Langone Hlth, NYU Grossman Sch Med, Dept Ophthalmol, New York, NY 10012 USAXinlei Wang - NYU, NYU Langone Hlth, NYU Grossman Sch Med, Dept Ophthalmol, New York, NY 10012 USAGadi Wollstein - NYU, NYU Langone Hlth, NYU Grossman Sch Med, Dept Ophthalmol, New York, NY 10012 USAJoel S. Schuman - Drexel UniversitySieun Lee - Simon Fraser Univ, Sch Engn Sci, Burnaby, BC, CanadaHaolun Shi - Simon Fraser Univ, Dept Stat & Actuarial Sci, Burnaby, BC, CanadaMyeong Jin Ju - Univ British Columbia, Dept Ophthalmol & Visual Sci, Vancouver, BC, CanadaJoanne Matsubara - Univ British Columbia, Dept Ophthalmol & Visual Sci, Vancouver, BC, CanadaMirza Faisal Beg - Simon Fraser Univ, Sch Engn Sci, Burnaby, BC, CanadaMarinko Sarunic - UCL, Inst Ophthalmol, London, EnglandRebecca M. Sappington - Wake Forest Univ, Sch Med, 1 Med Ctr Blvd, Winston Salem, NC 27157 USAKevin C. Chan - NYU, NYU Langone Hlth, NYU Grossman Sch Med, Dept Ophthalmol, New York, NY 10012 USA
- Publication Details
- Acta neuropathologica communications, v 12(1), pp 19-19
- Publisher
- Springer Nature
- Number of pages
- 16
- Grant note
- National Eye Institute; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Eye Institute (NEI)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:001155081900001
- Scopus ID
- 2-s2.0-85183709434
- Other Identifier
- 991021861208604721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Neurosciences