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Journal article
Effect of M1-M2 Polarization on the Motility and Traction Stresses of Primary Human Macrophages
Cellular and molecular bioengineering, v 9(3), pp 455-465
01 Sep 2016
PMID: 28458726
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Macrophages become polarized by cues in their environment and this polarization causes a functional change in their behavior. Two main subsets of polarized macrophages have been described. M1, or "classically activated" macrophages, are pro-inflammatory and M2, or "alternatively activated" macrophages, are anti-inflammatory. In this study, we investigated the motility and force generation of primary human macrophages polarized down the M1 and M2 pathways using chemokinesis assays and traction force microscopy on polyacrylamide gels. We found that M1 macrophages are significantly less motile and M2 macrophages are significantly more motile than unactivated M0 macrophages. We also showed that M1 macrophages generate significantly less force than M0 or M2 macrophages. We further found that M0 and M2, but not M1, macrophage force generation is dependent on ROCK signaling, as identified using the chemical inhibitor Y27632. Finally, using the chemical inhibitor blebbistatin, we found that myosin contraction is required for force generation by M0, M1, and M2 macrophages. This study represents the first investigation of the changes in the mechanical motility mechanisms used by macrophages after polarization.
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Details
- Title
- Effect of M1-M2 Polarization on the Motility and Traction Stresses of Primary Human Macrophages
- Creators
- Laurel E. Hind - University of PennsylvaniaEmily B. Lurier - Drexel UniversityMicah Dembo - Department of Biomedical Engineering Boston University Boston MAKara L. Spiller - Drexel UniversityDaniel A. Hammer - University of Pennsylvania
- Publication Details
- Cellular and molecular bioengineering, v 9(3), pp 455-465
- Publisher
- Springer Nature
- Number of pages
- 11
- Grant note
- GM1094287; HL18208 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA P01HL018208 / NATIONAL HEART, LUNG, AND BLOOD INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI) R01GM104287 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000381594700013
- Scopus ID
- 2-s2.0-84964344260
- Other Identifier
- 991019168590404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biophysics
- Cell & Tissue Engineering
- Cell Biology
- Engineering, Biomedical