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Effect of hepatic artery flow on bile secretory function after cold ischemia
Journal article   Peer reviewed

Effect of hepatic artery flow on bile secretory function after cold ischemia

David P Foley, Rocco Ricciardi, Angela N Traylor, Thomas J McLaughlin, Susan E Donohue, Suzanne M Wheeler, William C Meyers and Steven H Quarfordt
American journal of transplantation, v 3(2), pp 148-155
Feb 2003
PMID: 12603210

Abstract

Animals Bile - secretion Bilirubin - secretion Cholesterol - secretion Cryopreservation Female Hepatic Artery - physiopathology Ischemia - physiopathology Liver - blood supply Liver - drug effects Liver Transplantation - methods Liver Transplantation - physiology Reperfusion Injury - physiopathology Swine Taurocholic Acid - pharmacology Tissue Survival - physiology
These studies evaluated the influence of hepatic arterial flow on biliary secretion after cold ischemia. Preparation of livers for transplantation or hepatic support impairs biliary secretion. The earliest indication of cold preservation injury during reperfusion is circulatory function. Arterial flow at this time may be critical for bile secretion. Porcine livers were isolated, maintained at 4 degrees for 2 h and connected in an extracorporeal circuit to an anesthetized normal pig. The extracorporeal livers were perfused either by both the hepatic artery and portal vein (dual) or by the portal vein alone (single). Incremental doses of sodium taurocholate were infused into the portal vein of both the dual and single perfused livers, and the bile secretion was compared. Most endogenous bile acids are lost during hepatic isolation. After supplementation, the biliary secretion of phosphatidyl choline and cholesterol was significantly better in the dual than single vessel-perfused livers; however, no difference was seen in bilirubin output. Single perfused livers were completely unable to increase biliary cholesterol in response to bile acid. The dependence of bile cholesterol secretion on arterial flow indicates the importance of this flow to the detoxification of compounds dependent on phosphatidyl choline transport during early transplantation.

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Collaboration types
Domestic collaboration
Web of Science research areas
Surgery
Transplantation
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