Journal article
Effect of interleukin 1 beta on transducing mechanisms in 235-1 clonal pituitary cells
Biochemical and biophysical research communications, v 155(3), pp 1097-1104
1988
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Abstract
In the present study we investigated the effect of the interleukin 1 beta on intracellular free calcium concentrations in 235-1 cell line both in basal conditions and after stimulation by the calcium channel activator maitotoxin. Interleukin 1 beta (from 0.01 pM to 10 nM) was unable to significantly affect basal cytosolic free calcium levels in acute conditions. The preincubation of these cells with interleukin 1 beta for 48h modulates maitotoxin stimulation of calcium fluxes without modifying basal intracellular free calcium levels. Low concentrations of interleukin 1 beta (0.01 pM, 1 pM) caused a marked reduction of intracellular free calcium concentrations increase induced by maitotoxin while higher doses of the monokine potentiated maitotoxin stimulation of calcium fluxes. The specificity of interleukin 1 beta effect was tested by means of polyclonal anti-interleukin 1 beta antibody (titer 1:100) which significantly abolished the inhibitory effect of interleukin 1 beta on free cytosolic calcium levels. These results show that a long lasting interaction of interleukin 1 beta with its receptor is able to influence voltage-sensitive — calcium channels activation induced by maitotoxin in 235-1 cells.
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Details
- Title
- Effect of interleukin 1 beta on transducing mechanisms in 235-1 clonal pituitary cells
- Creators
- Gennaro Schettini - Dept. of Pharmacology, II School of Medicine, Via Pansini 5, 80131 Napoli, ITALYOlimpia Meucci - Dept. of Pharmacology, II School of Medicine, Via Pansini 5, 80131 Napoli, ITALYTullio Florio - Dept. of Pharmacology, II School of Medicine, Via Pansini 5, 80131 Napoli, ITALYGiuseppe Scala - Dept. of Biochemistry, II School of Medicine, Via Pansini 5, 80131 Napoli, ITALYElisa Landolfi - Dept. of Pharmacology, II School of Medicine, Via Pansini 5, 80131 Napoli, ITALYMaurizio Grimaldi - Dept. of Pharmacology, II School of Medicine, Via Pansini 5, 80131 Napoli, ITALY
- Publication Details
- Biochemical and biophysical research communications, v 155(3), pp 1097-1104
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:A1988Q239200002
- Scopus ID
- 2-s2.0-0023793191
- Other Identifier
- 991020111320604721
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Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biophysics