Journal article
Effect of membrane microheterogeneity and domain size on fluorescence resonance energy transfer
Biophysical journal, v 93(2), pp 655-667
15 Jul 2007
PMID: 17449659
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Studies of multicomponent membranes suggest lateral inhomogeneity in the form of membrane domains, but the size of small (nanoscale) domains in situ cannot be determined with current techniques. In this article, we present a model that enables extraction of membrane domain size from time-resolved fluorescence resonance energy transfer (FRET) data. We expand upon a classic approach to the infinite phase separation limit and formulate a model that accounts for the presence of disklike domains of finite dimensions within a two-dimensional infinite planar bilayer. The model was tested against off-lattice Monte Carlo calculations of a model membrane in the liquid-disordered (l(d)) and liquid-ordered (l(o)) coexistence regime. Simulated domain size was varied from 5 to 50 nm, and two fluorophores, preferentially partitioning into opposite phases, were randomly mixed to obtain the simulated time-resolved FRET data. The Monte Carlo data show clear differences in the efficiency of energy transfer as a function of domain size. The model fit of the data yielded good agreement for the domain size, especially in cases where the domain diameter is <20 nm. Thus, data analysis using the proposed model enables measurement of nanoscale membrane domains using time-resolved FRET.
Metrics
Details
- Title
- Effect of membrane microheterogeneity and domain size on fluorescence resonance energy transfer
- Creators
- Kevin B Towles - Department of Chemical and Biological Engineering, Drexel University, Philadelphia, Pennsylvania, USA. kbt22@drexel.eduAngela C BrownSteven P WrennNily Dan
- Publication Details
- Biophysical journal, v 93(2), pp 655-667
- Publisher
- United States
- Grant note
- R01 GM071355 / NIGMS NIH HHS R01GM071355 / NIGMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:000247465300030
- Scopus ID
- 2-s2.0-34447313944
- Other Identifier
- 991014877780404721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biophysics