Journal article
Effect of nerve regeneration factor on differentiation of PC12 cells and its signaling pathway
Progress in natural science, v 14(11), pp 971-974
01 Nov 2004
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The effects of nerve regeneration factor (NRF) on neuronal differentiation of PC12 cells and its signaling pathway are investigated by morphological observation and immunofluorescent cytochemical method, and the activity of ERK1/2 in NRF-treated PC12 cells in absence of serum is also studied by immuno- coprecipitation and Western blot analysis. The MEK1/2-specific inhibitor U0126, the broad-spectrum protein kinase C (PKC) inhibitor Goe6983 and tyrosine protein kinase (TPK) inhibitor genistein were used to determine the roles of the activation of ERK1/2 by NRF and the involvement of certain kinds of PKC or TPK receptor in this activation process. The results show that U0126 and Goe6983 inhibit the activation of ERK1/2 by NRF to different extents, while genistein has no effect on it, demonstrating that NRF remarkably induces neuronal differentiation of PC12 cells through activating ERK1/2 in a dose-dependent and time-dependent manner.
Metrics
Details
- Title
- Effect of nerve regeneration factor on differentiation of PC12 cells and its signaling pathway
- Creators
- Fei Ding - Nantong UniversityLiang Qiang - Nantong UniversityMei Liu - Nantong UniversityXingxing Gu - Nantong UniversityXiaosong Gu - Nantong University
- Publication Details
- Progress in natural science, v 14(11), pp 971-974
- Publisher
- Taylor and Francis
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000225308300006
- Scopus ID
- 2-s2.0-10444235669
- Other Identifier
- 991019201365704721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Materials Science, Multidisciplinary