Journal article
Effect of pH on the affinity of phospholipids for cholesterol
Lipids, v 24(5), pp 375-382
May 1989
PMID: 2755314
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The ability of multilamellar vesicles of phosphatidylcholine and phosphatidylethanolamine in aqueous phase to prevent access to cholesterol by a nonpolar solvent was examined. Phosphatidylethanolamine vesicles retained less sterol than phosphatidylcholine vesicles. In mixed vesicles, cholesterol was retained in proportion to the amount of phosphatidylcholine. To alter the charge and hydration of head groups, pH was adjusted from 1.2 to 12.5. Above pH 8, both phosphatidylethanolamine and phosphatidylcholine retained sterol in a 1∶1 molar ratio of phospholipid to cholesterol, regardless of acyl side chain composition. Between pH 2.0 and pH 8.0, sterol retention varied with type of head group and side chain. Lipids with 16‐carbon saturated side chains retained more sterol than 18‐carbon unsaturated or 12‐carbon saturated side chains. Between pH 1.1 and 2.0, none of the phosphatidylethanolamines retained sterol, but long chain phosphatidylcholines, saturated or unsaturated, retained sterol in a 1∶1 molar ratio of phospholipid to sterol. Short chain phosphatidylethanolamines and phosphatidylcholines retained 0 to 20% at the low‐ to mid‐pH range. Size of multilamellar vesicles, measured by Doppler effect light scattering analysis, had no bearing on sterol retention. Sonication of vesicles, which increases surface curvature, increases the retention of sterol. Fluorescence polarization indicated that cholesterol does not interact with DPPC or DLPC side chains. The observations can be interpreted in terms of space requirements of head groups, including charge repulsion and hydration. Other factors, such as monovalent cation replacement by protons, juxtaposition of charged groups on vesicle surfaces and length and unsaturation of acyl side chains affect the affinity of phospholipids for cholesterol.
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Details
- Title
- Effect of pH on the affinity of phospholipids for cholesterol
- Creators
- Myra K. Jacobsohn - Hahnemann University HospitalLinda S. Bazilian - Hahnemann University HospitalJanice Hardiman - Hahnemann University HospitalGert M. Jacobsohn - Hahnemann University Hospital
- Publication Details
- Lipids, v 24(5), pp 375-382
- Publisher
- Springer‐Verlag
- Number of pages
- 8
- Resource Type
- Journal article
- Language
- English
- Web of Science ID
- WOS:A1989U648400004
- Scopus ID
- 2-s2.0-0024389350
- Other Identifier
- 991019330799204721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Nutrition & Dietetics