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Effect of transgene copy number on survival in the G93A SOD1 transgenic mouse model of ALS
Journal article   Peer reviewed

Effect of transgene copy number on survival in the G93A SOD1 transgenic mouse model of ALS

Guillermo M Alexander, Kirsten L Erwin, Nathaniel Byers, Jeffrey S Deitch, Brian J Augelli, Elizabeth P Blankenhorn and Terry D Heiman-Patterson
Brain research. Molecular brain research., v 130(1)
2004
PMID: 15519671

Abstract

ALS G93A Transgene copy number Mice Real time PCR SOD1
Transgenic mice expressing multiple copies of the G93A mutant form of SOD1 develop motor neuron pathology and clinical symptoms similar to those seen in patients with amyotrophic lateral sclerosis (ALS). The phenotype of these mice is dependent on the number of transgene copies in their genome. Changes in transgene copy number, although rare, can sometimes occur while mating due to intra locus recombination events during meiosis. The objective of this study was to develop a real time quantitative PCR method to determine changes in transgene copy number in these mice and to evaluate the effect of transgene copy number on the phenotype of the G93A SOD1 mouse model of ALS.

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Collaboration types
Domestic collaboration
Web of Science research areas
Neurosciences
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