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Journal article
Effects of Acute Agonist Treatment on Subcellular Distribution of kappa Opioid Receptor in Rat Spinal Cord
Journal of neuroscience research, v 87(7), pp 1695-1702
15 May 2009
PMID: 19130621
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Abstract
We investigated whether acute treatment with agonists affected the subcellular distribution of K opioid receptor (KOPR) in the dorsal horn of the rat lumbar spinal cord by using immunoelectron microscopy. Rats were injected intrathecally (i.t.) with U50,488H (100 nmole), dynorphin A(1-17) (15 nmole), or vehicle. The doses chosen have been shown to induce antinociception. Rats were perfused transcardially 30 min later, and lumbar spinal cords were removed and processed for electron microscopic analysis. KOPR was stained with KT-2, a specific polyclonal antibody against the rat/mouse KOPR(371-380) peptide, followed by gold-labeled secondary antibody and silver intensification. The silver grains were present in axons, terminals, dendrites, and somata, and the association with plasma membranes was quantified in dendrites, because KOPR immunoreactivity was most frequently observed in these profiles. In vehicle-treated rats, similar to 27% of KOPR immunoreactivity was associated with plasma membranes. U50,488H, i.t., did not cause a significant change in the percentage of KOPR present on plasma membranes, whereas dynorphin A, i.t., significantly decreased cell surface KOPR to similar to 19%. In summary, these data indicate that U50,488H and dynorphin A differentially regulate the subcellular distribution of endogenous KOPR. (C) 2009 Wiley-Liss, Inc.
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Details
- Title
- Effects of Acute Agonist Treatment on Subcellular Distribution of kappa Opioid Receptor in Rat Spinal Cord
- Creators
- Yulin Wang - Temple UniversityWei Xu - Temple UniversityPeng Huang - Temple UniversityCharles Chavkin - University of WashingtonElisabeth J. Van Bockstaele - Jefferson Hospital for NeuroscienceLee-Yuan Liu-Chen - Temple University
- Publication Details
- Journal of neuroscience research, v 87(7), pp 1695-1702
- Publisher
- Wiley
- Number of pages
- 8
- Grant note
- R29DA009082 / NATIONAL INSTITUTE ON DRUG ABUSE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Drug Abuse (NIDA); European Commission P30 DA013429; DA17302; R01 DA009082; DA09082; R01 DA017302 / NIDA NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Drug Abuse (NIDA)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; Pharmacology and Physiology
- Web of Science ID
- WOS:000265312100019
- Scopus ID
- 2-s2.0-65849295964
- Other Identifier
- 991021903407004721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences