Journal article
Effects of Ca2+ sensing receptor activation in the growth plate
The FASEB journal, v 18(1), pp 143-145
Jan 2004
PMID: 14597561
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The Ca2+-sensing receptor (CaR) is a G protein-coupled receptor expressed in many mammalian tissues, including the long bone's growth plate. CaR knockout mice exhibit growth retardation, suggesting that CaR may promote skeletal growth. However, the complex phenotype of these knockout mice, which includes hyperparathyroidism, hypercalcemia, and hypophosphatemia, may confound the effects of CaR activation. To determine whether CaR regulates growth plate chondrogenesis and longitudinal bone growth, we chose an organ culture model. Fetal rat metatarsal bones (dpc 20) were cultured in serum-free medium for 7 days in the presence or absence of NPS-R-568, a CaR agonist. The addition of 10 nM NPS-R-568 increased the cumulative longitudinal growth of the metatarsal explants. To explore the underlying mechanisms, we then assessed the effects of NPS-R-568 on growth plate chondrocyte hypertrophy/differentiation and chondrocyte proliferation. After 7 days in culture, NPS-R-568 increased the height of the growth plate hypertrophic zone and the expression of collagen X, a marker of growth plate chondrocyte differentiation (assessed by immunohistochemistry). NPS-R-568 also induced a significant increase of the height of the growth plate proliferative zone and of the total thymidine incorporation in the metatarsal bone. In conclusion, our findings suggest that the activation of CaR in the growth plate accelerates longitudinal bone growth by stimulating growth plate chondrogenesis.
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Details
- Title
- Effects of Ca2+ sensing receptor activation in the growth plate
- Creators
- Shufang Wu - St. Christopher's Hospital for ChildrenTeresa Palese - University of Maryland, BaltimoreOm Prakash Mishra - St. Christopher's Hospital for ChildrenMaria Delivoria-Papadopoulos - St. Christopher's Hospital for ChildrenFrancesco De Luca - St. Christopher's Hospital for Children
- Publication Details
- The FASEB journal, v 18(1), pp 143-145
- Publisher
- Wiley
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics; College of Medicine
- Web of Science ID
- WOS:000188829300054
- Scopus ID
- 2-s2.0-1342332158
- Other Identifier
- 991019168315504721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biology
- Cell Biology