Journal article
Effects of DNA repair gene polymorphisms on DNA damage in human lymphocytes induced by a vinyl chloride metabolite in vitro
Biomarkers, v 19(4), pp 281-286
01 Jun 2014
PMID: 24731051
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Background: Epidemiologic studies suggest that variability in DNA damage from vinyl chloride monomer (VCM) may be partially mediated by genetic polymorphisms in DNA repair. This study aimed to corroborate these observations with controlled experiments in vitro using cell lines from individuals with differing DNA repair genotypes to determine damage following VCM metabolite exposure.
Methods: Matched pairs of lymphoblast cell lines (homozygous wild-type versus homozygous variant for either XRCC1 399 or XPD 751 polymorphism) were exposed to chloroacetaldehyde and analyzed by the cytokinesis-block micronucleus assay.
Results: All cell lines demonstrated a dose-response of increasing micronuclei with increasing exposure, but for both XRCC1 and XPD, the polymorphic cells peaked at higher micronucleus frequencies and declined at a slower rate to baseline than the wild-type cells.
Conclusion: This supports the findings that XRCC1 and XPD polymorphisms may result in deficient DNA repair of VCM-induced genetic damage.
Metrics
Details
- Title
- Effects of DNA repair gene polymorphisms on DNA damage in human lymphocytes induced by a vinyl chloride metabolite in vitro
- Creators
- Nannan Feng - Fudan UniversityYongliang Li - University of Illinois at ChicagoChangmin Long - University of Illinois at ChicagoZhao-lin Xia - Fudan UniversityPaul W. Brandt-Rauf - University of Illinois at Chicago
- Publication Details
- Biomarkers, v 19(4), pp 281-286
- Publisher
- Taylor & Francis
- Number of pages
- 6
- Grant note
- R01OH004192 / NATIONAL INSTITUTE FOR OCCUPATIONAL SAFETY AND HEALTH; United States Department of Health & Human Services; Centers for Disease Control & Prevention - USA; National Institute for Occupational Safety & Health (NIOSH) R01-OH04192 / NIOSH; United States Department of Health & Human Services; Centers for Disease Control & Prevention - USA; National Institute for Occupational Safety & Health (NIOSH)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000336730300004
- Scopus ID
- 2-s2.0-84901319549
- Other Identifier
- 991019176646704721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Toxicology