Journal article
Effects of Exendin-4 on the differentiation of neural stem cells from subventricular zone of adult mice in vitro
Zhongguo ying yong sheng li xue za zhi, v 35(3), pp 262-267
28 May 2019
PMID: 31257810
Abstract
To study the effect of exendin-4(Ex-4) on the differentiation of neural stem cells(NSCs) in adult mouse subventricular zone(SVZ)and its mechanism .
NSCs in the SVZ were derived from 5-week C57BL/6J mice and the expression of nestin was detected by immunofluorescence. The cell morphology was observed after the cells treatmed with 100 nmol/L Ex-4 for 14 days.The expressions of nestin and glucagon-like peptide-1 receptor (GLP-1R) were detected by immunofluorescence. GLP-1R was knocked down by using shRNA and the study was divided into four groups: control group, Ex-4 group, GLP-1R knockdown group, GLP-1R knockdown + Ex-4 group. After treatment with 100 nmol/L Ex-4 for 14 d, β-tublin III and glial fibrillary acidic protein (GFAP) were labeled by immunofluorescence and then the proportion of β-tublin III positive cells were counted. Western blot was used to detect the activation of cAMP-response element binding protein (CREB) in NSCs. In order to further study the effects of Ex-4 on mitogen-activated protein kinas
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Details
- Title
- Effects of Exendin-4 on the differentiation of neural stem cells from subventricular zone of adult mice in vitro
- Creators
- Fei Zhao - Graduate School of Jinzhou Medical University, Jinzhou 121000.Hui-You Xu - Center for Neurology and Neurosurgery of People's Armed Police Forces Medical Center, Tianjin 300162, China.Ke Ma - Center for Neurology and Neurosurgery of People's Armed Police Forces Medical Center, Tianjin 300162, China.Ji-Peng Jiang - Center for Neurology and Neurosurgery of People's Armed Police Forces Medical Center, Tianjin 300162, China.Jian Zhang - Center for Neurology and Neurosurgery of People's Armed Police Forces Medical Center, Tianjin 300162, China.Chen Dai - Center for Neurology and Neurosurgery of People's Armed Police Forces Medical Center, Tianjin 300162, China.Ying Jin - Center for Neurology and Neurosurgery of People's Armed Police Forces Medical Center, Tianjin 300162, China.Ping Li - Center for Neurology and Neurosurgery of People's Armed Police Forces Medical Center, Tianjin 300162, China.Hong-Tao Sun - Center for Neurology and Neurosurgery of People's Armed Police Forces Medical Center, Tianjin 300162, China.Zhen-Guo Wang - Center for Neurology and Neurosurgery of People's Armed Police Forces Medical Center, Tianjin 300162, ChinaXu-Yi Chen - Center for Neurology and Neurosurgery of People's Armed Police Forces Medical Center, Tianjin 300162, China.
- Publication Details
- Zhongguo ying yong sheng li xue za zhi, v 35(3), pp 262-267
- Resource Type
- Journal article
- Language
- Chinese
- Academic Unit
- Neurobiology and Anatomy
- Scopus ID
- 2-s2.0-85068981757
- Other Identifier
- 991020545124604721