Journal article
Effects of Gelatin Methacrylate Hydrogel on Corneal Repair and Regeneration in Rats
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY, v 10(14), 25
Dec 2021
PMID: 34935910
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Purpose: This study investigates the repairing process of rat cornea after surgery of lamellar keratoplasty (LKP) and evaluates the effects of gelatin methacrylate (GelMA) hydrogel.Methods: In the LKP group, the lamellar stroma matrixes of Sprague-Dawley rats were transplanted to enhanced green fluorescent protein rats, whereas those in the GelMA group were also embedded with a GelMA hydrogel during the corneal transplantation. Grafted eyes were harvested on days seven, 30, and 90. Hematoxylin and eosin staining, immunofluorescence staining, scanning electron microscopy, optical coherence tomog-raphy, and a slit-lamp microscope were used to study the process of corneal restoration and regeneration.Results: A total of 42 rats were analyzed, including 18 rats in each of the experimen-tal group and six rats in the control group. After three months, the infiltration degree of inflammatory cells differed between the LKP group and the GelMA group (P < 0.001). Moreover, in multiple comparisons in corneal thickness, significant difference was observed between the LKP group and the GelMA group. There was also divergence in the results between the LKP group and the control group (P < 0.001, P < 0.001). At the same time, the expression of alpha-smooth muscle actin (alpha-SMA) and transforming growth factor (TGF)-)51 varied distinctly between the LKP group and the GelMA group (P < 0.05, P <0.001).Conclusions: Significant differences were demonstrated between the LKP group and the GelMA group in inflammatory cell infiltration, corneal thickness, as well as the expression of alpha-SMA and TGF-)51. Those differences indicate the ability of GelMA hydro-gel to support alleviation in corneal stroma fibrosis and show the influences of fibrosis in the dysfunction of corneal refractive power.Translational Relevance: Our research provides new ideas for the future development of LKP and tissue-engineered corneas.
Metrics
Details
- Title
- Effects of Gelatin Methacrylate Hydrogel on Corneal Repair and Regeneration in Rats
- Publication Details
- TRANSLATIONAL VISION SCIENCE & TECHNOLOGY, v 10(14), 25
- Publisher
- ASSOC RESEARCH VISION OPHTHALMOLOGY INC; ROCKVILLE
- Grant note
- Supported by the National Key Research and Development Program of China (No.2018YFC1106000) , the Key Research and Devel-opment Projects of People?s Liberation Army (No. BWS17J036) , and the Project of Basic Research of Shenzhen, China (JCYJ20170412101508433 &JCYJ20180507183655307) .
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Drexel University
- Web of Science ID
- WOS:000877998400001
- Scopus ID
- 2-s2.0-85122904024
- Other Identifier
- 991021860756004721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Ophthalmology