Journal article
Effects of Interleukin-10 (IL-10) on Pain Behavior and Gene Expression Following Excitotoxic Spinal Cord Injury in the Rat
Experimental neurology, v 168(1)
Mar 2001
PMID: 11170729
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Intraspinal injection of quisqualic acid (QUIS) produces excitotoxic injury with pathophysiological characteristics similar to those associated with ischemic and traumatic spinal cord injury (SCI). Responses to QUIS-induced injury include an inflammatory component, as well as the development of spontaneous and evoked pain behaviors. We hypothesized that QUIS-induced inflammation and subsequent gene expression contribute to the development and progression of pain-related behaviors and that blockade of inflammation-related gene expression leads to the amelioration of these behaviors. Using the QUIS model of spinal cord injury, we examined whether interleukin-10 (IL-10), a potent anti-inflammatory cytokine, is able to reduce mRNA levels of inflammatory and cell death-related genes leading to a reduction of pain behaviors. The results demonstrate that animals receiving systemic injection of IL-10, 30 minutes following QUIS-induced SCI, showed a significant delay in the onset of excessive grooming behavior, a significant reduction in grooming severity, and a significant reduction in the longitudinal extent of a pattern of neuronal loss within the spinal cord characterized as “grooming-type damage.” QUIS injections also resulted in an increase in mRNA levels of interleukin-1 beta (IL-1β), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), CD95 ligand (CD95-L, also called FAS-L/APO-1L), and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). Results of QUIS injury plus IL-10 treatment resulted in a significant downregulation of IL1-β and iNOS mRNA and these results were supported by Western blot analysis of protein levels following IL-10 treatment. These data suggest that IL-10 reduces inflammation and that targeting injury-induced inflammation is an effective strategy for limiting the extent of neuronal damage following excitotoxic SCI and thus the onset and progression of injury-induced pain behaviors.
Metrics
Details
- Title
- Effects of Interleukin-10 (IL-10) on Pain Behavior and Gene Expression Following Excitotoxic Spinal Cord Injury in the Rat
- Creators
- Jeffery A. Plunkett - University of MiamiChen-Guang Yu - University of MiamiJulia M. Easton - University of MiamiJohn R. Bethea - University of MiamiRobert P. Yezierski - University of Miami
- Publication Details
- Experimental neurology, v 168(1)
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology; College of Arts and Sciences; Drexel University
- Web of Science ID
- WOS:000167202400013
- Scopus ID
- 2-s2.0-0035126792
- Other Identifier
- 991020112275004721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Neurosciences