Journal article
Effects of Src kinase inhibition on expression of pro-caspase-2 after brain hypoxia in a piglet animal model
Neuroreport, v 28(12), pp 770-773
16 Aug 2017
PMID: 28658050
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Caspase-2 has features of both initiator and effector caspases. Previously, we have shown that brain hypoxia-induced production of caspases 1, 3, 8, and 9 is Src kinase mediated, a nonreceptor intracellular family of kinases. The present study tests the hypothesis that hypoxia results in increased expression of caspase-2 and this effect is mediated by Src kinase. Two to three days old newborn piglets were subjected to normoxia, hypoxia (Hx, FiO(2) 7%), and Src kinase inhibition (using PP2, 1mg/kg, intravenous), followed by 30min of acute hypoxia (Hx+PP2). ATP and phosphocreatine were determined biochemically to verify energy molecule depletion in the hypoxic groups. The cytosolic brain function was isolated and a western blot analysis was carried out using an antibody specific for the caspase-2. The immune-complex band density was expressed as OD/mm(2). Caspase-2 expression was increased two-fold in the Hx group. After Src kinase inhibition followed by hypoxia, caspase-2 expression was similar to normoxia levels. We conclude that hypoxia results in increased expression of caspase-2 protein in the cytosolic fraction of the cerebral cortex of the newborn piglets. This increase is mediated by Src kinase.
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Details
- Title
- Effects of Src kinase inhibition on expression of pro-caspase-2 after brain hypoxia in a piglet animal model
- Creators
- Dimitrios Angelis - Texas Tech University Health Sciences CenterMaria Delivoria-Papadopoulos - Drexel University
- Publication Details
- Neuroreport, v 28(12), pp 770-773
- Publisher
- Lippincott Williams & Wilkins
- Number of pages
- 4
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics
- Web of Science ID
- WOS:000405091000012
- Scopus ID
- 2-s2.0-85021390425
- Other Identifier
- 991019169680704721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences