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Effects of crowders on the equilibrium and kinetic properties of protein aggregation
Journal article   Peer reviewed

Effects of crowders on the equilibrium and kinetic properties of protein aggregation

John Bridstrup and Jian-Min Yuan
Chemical physics letters, v 659, pp 252-257
16 Aug 2016

Abstract

Chemistry Chemistry, Physical Physical Sciences Physics Physics, Atomic, Molecular & Chemical Science & Technology
The equilibrium and kinetic properties of protein aggregation systems in the presence of crowders are investigated using simple, illuminating models based on mass-action laws. Our model yields analytic results for equilibrium properties of protein aggregates, which fit experimental data of actin and ApoC-II with crowders reasonably well. When the effects of crowders on rate constants are considered, our kinetic model is in good agreement with experimental results for actin with dextran as the crowder. Furthermore, the model shows that as crowder volume fraction increases, the length distribution of fibrils becomes narrower and shifts to shorter values due to volume exclusion. (C) 2016 Elsevier B.V. All rights reserved.

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Chemistry, Physical
Physics, Atomic, Molecular & Chemical
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