Journal article
Effects of dispensing pressure and nozzle diameter on cell survival from solid freeform fabrication-based direct cell writing
Tissue engineering. Part A, v 14(1), pp 41-48
01 Jan 2008
PMID: 18333803
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Novel technologies are emerging that incorporate cells as part of the building blocks for various biomanufacturing processes, such as solid freeform fabricated tissue constructs for tissue regeneration, three-dimensional pharmacokinetic models, cell-based microelectromechanical systems, sensors, and microfluidic devices. However, the effects of these biomanufacturing processes on cells have not been fully studied. This paper examines the effect of solid freeform fabrication-based direct cell writing process, focusing on dispensing pressure and nozzle size, on the viability and functional behavior of HepG2 cells encapsulated within alginate. Our experimental results revealed a process-induced mechanical damage to cell membrane integrity, causing a quantifiable loss in cell viability due to incremental increases and decreases in the studied process parameters of dispensing pressure and nozzle size, respectively. The experimental results also suggested that cells may require a recovery period following direct cell writing biofabrication. The general finding of this study may be applicable to freeform fabrication of cell-based tissue constructs and three-dimensional biological models.
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Details
- Title
- Effects of dispensing pressure and nozzle diameter on cell survival from solid freeform fabrication-based direct cell writing
- Creators
- Robert Chang - Drexel UniversityWei Sun - Drexel University
- Publication Details
- Tissue engineering. Part A, v 14(1), pp 41-48
- Publisher
- Mary Ann Liebert Inc
- Number of pages
- 8
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000253960900005
- Scopus ID
- 2-s2.0-38349103640
- Other Identifier
- 991019167699604721
UN Sustainable Development Goals (SDGs)
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Cell & Tissue Engineering
- Cell Biology
- Engineering, Biomedical
- Materials Science, Biomaterials