Journal article
Effects of divalent cations on snake venom cardiotoxin-induced hemolysis and 3H-deoxyglucose-6-phosphate release from human red blood cells
Toxicon (Oxford), v 27(12), pp 1297-1305
1989
PMID: 2629171
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Abstract
Ming-Shi Jiang,
J. E. Fletcher and
L. A. Smith. Effects of divalent cations on snake venom cardiotoxin-induced hemolysis and
3H-deoxyglucose-6-phosphate release from human red blood cells.
Toxicon
27, 1297–1305, 1989.—At a low concentration of
Naja naja kaouthia cardiotoxin (3 μM) Ca
2+, Sr
2+ and Ba
2+ (2 mM), had little to no effect on
3H-deoxyglucose-6-phosphate (
3H-dGlu-6-p) or hemoglobin release. At higher concentrations of
N. n. kaouthia cardiotoxin (≥ 10
μM), Ca
2+ (2 mM), but not Sr
2+ or Ba
2+, significantly enhanced
3H-dGlu-6-p and hemoglobin release. Mn
2+ (2 mM) almost completely inhibited
3H-dGlu-6-p release and hemolysis at both the 3 μM and 10 μM concentrations of cardiotoxin. At a fixed concentration of
N. n. kaouthia cardiotoxin (3 μM), Ca
2+ at low concentrations (0.5 mM) enhanced
3H-dGlu-6-p and hemoglobin release, but at higher concentrations caused a dose-dependent inhibition of cardiotoxin action. The cardiotoxin from
N. n. kaouthia venom (3 μM) induced
3H-dGlu-6-p release and hemolysis release with similar time courses and to similar extents.
3H-dGlu-6-p release induced by cardiotoxin was greatly enhanced as the pH of the medium was increased from 7.0 to 8.5. Similarities between
3H-dGlu-6-p and hemoglobin release do not support opening of pores in the plasmalemma of all red blood cells as the mode of action of cardiotoxins, but suggests that complete lysis of a subpopulation of cells occurs. Cardiotoxins have two components of lysis, only one of which is Ca
2+-dependent. The Ca
2+-dependent lysis is only evident at higher cardiotoxin concentrations and is likely due to trace phospholipase A
2 contamination in the toxin fraction. Mn
2+ is an effective antagonist of cardiotoxin action.
Metrics
Details
- Title
- Effects of divalent cations on snake venom cardiotoxin-induced hemolysis and 3H-deoxyglucose-6-phosphate release from human red blood cells
- Creators
- Ming-Shi Jiang - Hahnemann University HospitalJeffrey E. Fletcher - Hahnemann University HospitalLeonard A. Smith - Department of Toxinology, Pathology Division, USAMRIID, Fort Detrick, MD 21701, U.S.A.Leanice A Smith - Counseling and Family Therapy
- Publication Details
- Toxicon (Oxford), v 27(12), pp 1297-1305
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Counseling and Family Therapy
- Web of Science ID
- WOS:A1989CJ64300006
- Scopus ID
- 2-s2.0-0024935807
- Other Identifier
- 991019183958204721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Pharmacology & Pharmacy
- Toxicology