Journal article
Effects of host homogenate on the motility of endozoic algae from Cassiopiea xamachana
American zoologist, Vol.31(5), pp.145A-145A
01 Jan 1991
Abstract
Zooxanthellae (from Cassiopiea xamachana ) cultured on a 14:10 hr light:dark cycle demonstrate a diel pattern of cell motility and phased cell division reflective of the light:dark cycle. Dividing cells are typically found as doublets which peak late in the dark period; motile cells are observed one hour after the onset of the light period with a motility peak three to six hours later. This pattern of cell motility is significantly inhibited when cultures are exposed to low concentrations (50 mu g protein/ml) of host tissue homogenate at the onset of the dark period, an effect not observed with treatment at the onset of the light period or during the motility peak. This suggests that the homogenate activity effects some process which occurs in the dark period prior to flagellar development and movement. Size exclusion fractionation of host homogenate demonstrated that active component(s) fall within the 100-300 Kd molecular weight range. Subsequent analysis by HPLC showed that activity was confined to several distinct fractions. The suppression of motility is mimicked by the microtubule inhibitor griseofulvin, prompting a current investigation of the effect of homogenate fractions on cell division and microtubule assembly.
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Details
- Title
- Effects of host homogenate on the motility of endozoic algae from Cassiopiea xamachana
- Creators
- J CliffordR Blanquet
- Publication Details
- American zoologist, Vol.31(5), pp.145A-145A
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology; [Retired Faculty]
- Identifiers
- 991020202229504721