Journal article
Effects of injury size on local and systemic immune cell dynamics in volumetric muscle loss
npj Regenerative medicine, v 10(1), 9
13 Feb 2025
PMID: 39939310
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We took a systems approach to the analysis of macrophage phenotype in regenerative and fibrotic volumetric muscle loss outcomes in mice together with analysis of systemic inflammation and of other leukocytes in the muscle, spleen, and bone marrow. Differences in expression of macrophage phenotype markers occurred as early as day 1, persisted to at least day 28, and were associated with increased numbers of leukocytes in the muscle and bone marrow, increased pro-inflammatory marker expression in splenic macrophages, and changes in the levels of pro-inflammatory cytokines in the blood. The most prominent differences were in muscle neutrophils, which were much more abundant in fibrotic outcomes compared to regenerative outcomes at day 1 after injury. However, neutrophil depletion had little to no effect on macrophage phenotype or on muscle repair outcomes. Together, these results suggest that the entire system of immune cell interactions must be considered to improve muscle repair outcomes.
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Details
- Title
- Effects of injury size on local and systemic immune cell dynamics in volumetric muscle loss
- Creators
- Ricardo Whitaker - Drexel UniversitySamuel Sung - Drexel UniversityTina Tylek - Drexel UniversityGregory E Risser - Drexel UniversityErin M O'Brien - Drexel UniversityPhoebe Ellin Chua - Drexel UniversityThomas Li - Drexel UniversityRyan J Petrie - Drexel UniversityLin Han - Drexel UniversityBenjamin I Binder-Markey - Drexel UniversityKara L Spiller - Drexel University
- Publication Details
- npj Regenerative medicine, v 10(1), 9
- Publisher
- Springer Nature
- Number of pages
- 14
- Grant note
- AR083080 / U.S. Department of Health & Human Services | NIH | National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) AI045008 / Division of Intramural Research, National Institute of Allergy and Infectious Diseases (Division of Intramural Research of the NIAID) R01 HL130037 / NHLBI NIH HHS F31 HL158189 / NHLBI NIH HHS P30 AI045008 / NIAID NIH HHS R21 AR083080 / NIAMS NIH HHS P30 CA056036 / NCI NIH HHS HL130037 / U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute (NHLBI) P30 CA016520 / NCI NIH HHS HL158189 / U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute (NHLBI)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology; School of Biomedical Engineering, Science, and Health Systems; Chemical and Biological Engineering; Physical Therapy (and Rehabilitation Sciences)
- Web of Science ID
- WOS:001419154100001
- Scopus ID
- 2-s2.0-85218336233
- Other Identifier
- 991022028137804721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Cell & Tissue Engineering
- Engineering, Biomedical