Journal article
Effects of low AIPL1 expression on phototransduction in rods
Investigative ophthalmology & visual science, v 47(5), pp 2185-2194
01 May 2006
PMID: 16639031
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
To investigate the impact of aryl hydrocarbon receptor-interacting protein-like (AIPL)-1 on photoreception in rods.
Photoresponses of mouse rods expressing lowered amounts of AIPL1 were studied by single-cell and electroretinogram (ERG) recordings. Phototransduction protein levels and enzymatic activities were determined in biochemical assays. Ca2+ dynamics were probed with a fluorescent dye. Comparisons were made to rods expressing mutant Y99C guanylate cyclase activating protein (GCAP)-1, to understand which effects arose from elevated dark levels of cGMP and Ca2+.
Except for PDE, transduction protein levels were normal in low-AIPL1 retinas, as were guanylate cyclase (GC), rhodopsin kinase (RK), and normalized phosphodiesterase (PDE) activities. Y99C and low-AIPL1 rods were more sensitive to flashes than normal, but flash responses of low-AIPL1 rods showed an abnormal delay, reduced rate of increase, and longer recovery not present in Y99C rod responses. In addition, low-AIPL1 rods but not Y99C rods failed to reach the normal light-induced minimum in Ca2+ concentration.
Reduced AIPL1 delayed the photoresponse, decreased its amplification constant, slowed a rate-limiting step in its recovery, and limited the light-induced decrease in Ca2+. Not all changes were attributable to decreased PDE or to elevated cGMP and Ca2+ in darkness. Therefore, AIPL1 directly or indirectly affects more than one component of phototransduction.
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Details
- Title
- Effects of low AIPL1 expression on phototransduction in rods
- Creators
- Clint L Makino - Massachusetts Eye and Ear InfirmaryXiao-Hong WenNorman MichaudIgor V Peshenko - Salus UniversityBasil Pawlyk - Massachusetts Eye and Ear InfirmaryRichard S Brush - University of OklahomaMaria SolovievXiaoqing Liu - Massachusetts Eye and Ear InfirmaryMichael L Woodruff - University of California, Los AngelesPeter D CalvertAndrey B Savchenko - Salus UniversityRobert E Anderson - University of OklahomaGordon L Fain - University of California, Los AngelesTiansen Li - Massachusetts Eye and Ear InfirmaryMichael A Sandberg - Massachusetts Eye and Ear InfirmaryAlexander M Dizhoor - Salus University
- Publication Details
- Investigative ophthalmology & visual science, v 47(5), pp 2185-2194
- Publisher
- ARVO
- Grant note
- R01 EY004149 / NEI NIH HHS EY01844 / NEI NIH HHS P30 EY012190 / NEI NIH HHS P30 EY014104 / NEI NIH HHS EY12190 / NEI NIH HHS R01 EY012944 / NEI NIH HHS EY04149 / NEI NIH HHS EY00871 / NEI NIH HHS EY11522 / NEI NIH HHS EY10309 / NEI NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy; Pennsylvania College of Optometry (PCO)
- Web of Science ID
- WOS:000237451100060
- Scopus ID
- 2-s2.0-33744766769
- Other Identifier
- 991022035259004721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Ophthalmology