Journal article
Effects of non-thermal plasma on mammalian cells
PloS one, v 6(1), pp e16270-e16270
21 Jan 2011
PMID: 21283714
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Thermal plasmas and lasers have been widely used in medicine to cut, ablate and cauterize tissues through heating; in contrast, non-thermal plasma produces no heat, so its effects can be selective. In order to exploit the potential for clinical applications, including wound healing, sterilization, blood coagulation, and cancer treatment, a mechanistic understanding of the interaction of non-thermal plasma with living tissues is required. Using mammalian cells in culture, it is shown here that non-thermal plasma created by dielectric barrier discharge (DBD) has dose-dependent effects that range from increasing cell proliferation to inducing apoptosis. It is also shown that these effects are primarily due to formation of intracellular reactive oxygen species (ROS). We have utilized γ-H2AX to detect DNA damage induced by non-thermal plasma and found that it is initiated by production of active neutral species that most likely induce formation of organic peroxides in cell medium. Phosphorylation of H2AX following non-thermal plasma treatment is ATR dependent and ATM independent, suggesting that plasma treatment may lead to replication arrest or formation of single-stranded DNA breaks; however, plasma does not lead to formation of bulky adducts/thymine dimers.
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Details
- Title
- Effects of non-thermal plasma on mammalian cells
- Creators
- Sameer Kalghatgi - Department of Electrical and Computer Engineering, Drexel University, Philadelphia, Pennsylvania, United States of AmericaCrystal M KellyEkaterina CercharBehzad TorabiOleg AlekseevAlexander FridmanGary FriedmanJane Azizkhan-Clifford
- Publication Details
- PloS one, v 6(1), pp e16270-e16270
- Publisher
- Public LIbrary of Science (PLOS); United States
- Grant note
- R01 EB013011 / NIBIB NIH HHS R01 EB013011-01 / NIBIB NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology; Electrical and Computer Engineering; Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000286522300026
- Scopus ID
- 2-s2.0-79251617843
- Other Identifier
- 991014877682704721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Physics, Fluids & Plasmas