Journal article
Effects of residual antibiotics in groundwater on Salmonella typhimurium: changes in antibiotic resistance, in vivo and in vitro pathogenicity
Journal of environmental monitoring, v 14(1), 41
Jan 2012
PMID: 22051852
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
An outbreak-causing strain of Salmonella enterica serovar Typhimurium was exposed to groundwater with residual antibiotics for up to four weeks. Representative concentrations (0.05, 1, and 100 μg L(-1)) of amoxicillin, tetracycline, and a mixture of several other antibiotics (1 μg L(-1) each) were spiked into artificially prepared groundwater (AGW). Antibiotic susceptibility analysis and the virulence response of stressed Salmonella were determined on a weekly basis by using human epithelial cells (HEp2) and soil nematodes (C. elegans). Results have shown that Salmonella typhimurium remains viable for long periods of exposure to antibiotic-supplemented groundwater; however, they failed to cultivate as an indication of a viable but nonculturable state. Prolonged antibiotics exposure did not induce any changes in the antibiotic susceptibility profile of the S. typhimurium strain used in this study. S. typhimurium exposed to 0.05 and 1 μg L(-1) amoxicillin, and 1 μg L(-1) tetracycline showed hyper-virulent profiles in both in vitro and in vivo virulence assays with the HEp2 cells and C. elegans respectively, most evident following 2nd and 3rd weeks of exposure.
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Details
- Title
- Effects of residual antibiotics in groundwater on Salmonella typhimurium: changes in antibiotic resistance, in vivo and in vitro pathogenicity
- Creators
- Berat Z Haznedaroglu - Yale UniversityMarylynn V Yates - University of California, RiversideMorris F Maduro - University of California, RiversideSharon L Walker - University of California, Riverside
- Publication Details
- Journal of environmental monitoring, v 14(1), 41
- Publisher
- Royal Society of Chemistry
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Engineering
- Web of Science ID
- WOS:000298855800005
- Scopus ID
- 2-s2.0-84855384089
- Other Identifier
- 991021229879804721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Chemistry, Analytical
- Environmental Sciences