Journal article
Effects of the expression level of epidermal growth factor receptor on the ligand-induced restructuring of focal adhesions: a QCM-D study
Analytical and bioanalytical chemistry, v 405(4), pp 1153-1158
2013
PMID: 23180089
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Epidermal growth factor receptor (EGFR) plays a major role in cell migration and invasion and is considered to be the primary source of activation of various malignant tumors. To gain insight into how elevated levels of EGFR influence cellular function, particularly cell motility, we used a quartz crystal microbalance with dissipation monitoring (QCM-D) to examine restructuring of focal adhesions in MCF-10A cells induced by epidermal growth factor. Engineered cells that overexpress epidermal growth factor receptor (EGFR) exhibited a very different kinetic profile from wildtype MCF-10A cells that have a lower level of EGFR with a higher rate for the initial disassembly of focal adhesion and a much lower rate for the later reassembly of focal adhesions. It is conceivable that these effects exhibited by EGFR-overexpressing cells may promote the initiation and maintenance of a more favorable adhesion state for cell migration. This study has demonstrated the capability of the dissipation monitoring function of the QCM-D to quantitatively assess kinetic aspects of cellular processes with a high temporal resolution and sensitivity.
Figure
Characterization of the effects of the expression level of epidermal growth factor receptor on the kinetics of the epidermal growth factor-induced restructuring of focal adhesions with the quartz crystal microbalance with dissipation monitoring.
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Details
- Title
- Effects of the expression level of epidermal growth factor receptor on the ligand-induced restructuring of focal adhesions: a QCM-D study
- Creators
- Marcela P. Garcia - Drexel UniversityAmmar Shahid - Drexel UniversityJennifer Y. Chen - Drexel UniversityJun Xi - Drexel University
- Publication Details
- Analytical and bioanalytical chemistry, v 405(4), pp 1153-1158
- Publisher
- Springer-Verlag
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:000313735000002
- Scopus ID
- 2-s2.0-84873747310
- Other Identifier
- 991019169659204721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical