Efficacy of post-harvest rinsing and bleach disinfection of E. coli O157:H7 on spinach leaf surfaces

Nichola M Kinsinger; Holly M Mayton; Madeline R Luth; Sharon L Walker

doi:10.1016/j.fm.2016.10.019

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Efficacy of post-harvest rinsing and bleach disinfection of E. coli O157:H7 on spinach leaf surfaces

Journal article

Open access

Peer reviewed

Efficacy of post-harvest rinsing and bleach disinfection of E. coli O157:H7 on spinach leaf surfaces

Nichola M Kinsinger, Holly M Mayton, Madeline R Luth and Sharon L Walker

Food microbiology, v 62, pp 212-220

Apr 2017

DOI: https://doi.org/10.1016/j.fm.2016.10.019

PMID: 27889151

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Abstract

Bacterial Adhesion - drug effects

Bacterial Load

Colony Count, Microbial

Disinfectants - pharmacology

Disinfection - methods

Disinfection - standards

Escherichia coli O157 - drug effects

Escherichia coli O157 - growth & development

Escherichia coli O157 - metabolism

Escherichia coli O157 - ultrastructure

Food Microbiology

Kinetics

Microscopy, Electron, Scanning

Plant Leaves - chemistry

Plant Leaves - microbiology

Plant Leaves - ultrastructure

Sodium Hypochlorite - pharmacology

Spinacia oleracea - microbiology

Water

Attachment and detachment kinetics of Escherichia coli O157:H7 from baby spinach leaf epicuticle layers were investigated using a parallel plate flow chamber. Mass transfer rate coefficients were used to determine the impact of water chemistry and common bleach disinfection rinses on the removal and inactivation of the pathogen. Attachment mass transfer rate coefficients generally increased with ionic strength. Detachment mass transfer rate coefficients were nearly the same in KCl and AGW rinses; however, the detachment phase lasted longer in KCl than AGW (18 ± 4 min and 4 ± 2 min, respectively), indicating that the ions present during attachment play a significant role in the cells' ability to remain attached. Specifically, increasing bleach rinse concentration by two orders of magnitude was found to increase the detachment mass transfer rate coefficient by 20 times (from 5.7 ± 0.7 × 10 m/s to 112.1 ± 26.8 × 10 m/s for 10 ppb and 1000 ppb, respectively), and up to 88 ± 4% of attached cells remained alive. The spinach leaf texture was incorporated within a COMSOL model of disinfectant concentration gradients, which revealed nearly 15% of the leaf surface is exposed to almost 1000 times lower concentration than the bulk rinse solution.

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15 citations in Web of Science

17 citations in Scopus

Details

Title: Efficacy of post-harvest rinsing and bleach disinfection of E. coli O157:H7 on spinach leaf surfaces
Creators: Nichola M Kinsinger - University of California, Riverside
Holly M Mayton - University of California, Riverside
Madeline R Luth - University of California, Riverside
Sharon L Walker - University of California, Riverside
Publication Details: Food microbiology, v 62, pp 212-220
Publisher: Elsevier
Resource Type: Journal article
Language: English
Academic Unit: College of Engineering
Web of Science ID: WOS:000390499900030
Scopus ID: 2-s2.0-84992187390
Other Identifier: 991021229983604721

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Web of Science research areas: Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology