Journal article
Efficient Site-Specific Nonribozyme Opening of Hepatitis Delta Virus Genomic RNA in Infected Livers
Journal of virology, v 74(21), pp 9889-9894
01 Nov 2000
PMID: 11024115
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
ABSTRACT
Examination of the 1,679-nucleotide (nt) unit-length hepatitis delta virus (HDV) RNAs in the livers of two HDV-infected woodchucks showed that 96% of the antigenomic RNA but only 50% of the genomic RNA was circular. We subsequently found that at least half of the linear unit-length genomic RNA was open at a unique location. Using a modified form of RNA ligation-mediated amplification of cDNA ends, we showed that the 5′ end was located at nt 1212. Like the previously described ribozyme cleavage site at nt 686, the new site produced a 5′-OH. Nevertheless, we showed that this novel site was not produced by activity of the HDV genomic ribozyme. We speculate that the 5′ end at nt 1212 reflects a preferred site of posttranscriptional endonucleolytic cleavage of genomic RNA.
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Details
- Title
- Efficient Site-Specific Nonribozyme Opening of Hepatitis Delta Virus Genomic RNA in Infected Livers
- Creators
- Jinhong Chang - Fox Chase Cancer CenterGloria Moraleda - Fox Chase Cancer CenterSeverin Gudima - Fox Chase Cancer CenterJohn Taylor - Fox Chase Cancer Center
- Publication Details
- Journal of virology, v 74(21), pp 9889-9894
- Publisher
- American Society for Microbiology (ASM)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000089819900009
- Scopus ID
- 2-s2.0-0033780149
- Other Identifier
- 991020547606504721
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- Web of Science research areas
- Virology