Efficient and specific removal of albumin from human serum samples

Laura F Steel; Michael G Trotter; Pamela B Nakajima; Taj S Mattu; Gregory Gonye; Timothy Block

doi:10.1074/mcp.M300026-MCP200

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Efficient and specific removal of albumin from human serum samples

Journal article

Open access

Peer reviewed

Efficient and specific removal of albumin from human serum samples

Laura F Steel, Michael G Trotter, Pamela B Nakajima, Taj S Mattu, Gregory Gonye and Timothy Block

Molecular & cellular proteomics, Vol.2(4)

Apr 2003

DOI: https://doi.org/10.1074/mcp.M300026-MCP200

PMID: 12754305

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Abstract

Triazines

Amino Acid Sequence

Immunoglobulin G - isolation & purification

Coloring Agents

Humans

Serum Albumin - isolation & purification

Blood Protein Electrophoresis

Molecular Sequence Data

Electrophoresis, Gel, Two-Dimensional

Antibodies, Monoclonal

Bacterial Proteins

Serum Albumin - immunology

Peptide Mapping

Proteomics

Serum

Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Polymers

Serum Albumin - chemistry

Patient serum or plasma is frequently monitored for biochemical markers of disease or physiological status. Many of the rapidly evolving technologies of proteome analysis are being used to find additional clinically informative protein markers. The unusually high abundance of albumin in serum can interfere with the resolution and sensitivity of many proteome profiling techniques. We have used monoclonal antibodies against human serum albumin (HSA) to develop an immunoaffinity resin that is effective in the removal of both full-length HSA and many of the HSA fragments present in serum. This resin shows markedly better performance than dye-based resins in terms of both the efficiency and specificity of albumin removal. Immunoglobulins are another class of highly abundant serum protein. When protein G resin is used together with our immunoaffinity resin, Ig proteins and HSA can be removed in a single step. This strategy could be extended to the removal of any protein for which specific antibodies or affinity reagents are available.

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Title: Efficient and specific removal of albumin from human serum samples
Creators: Laura F Steel - Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Jefferson Center for Biomedical Research, Doylestown, Pennsylvania 18901, USA. laura.steel@jefferson.edu
Michael G Trotter
Pamela B Nakajima
Taj S Mattu
Gregory Gonye
Timothy Block
Publication Details: Molecular & cellular proteomics, Vol.2(4)
Publisher: ASBMB Publications / Elsevier; United States
Resource Type: Journal article
Language: English
Academic Unit: Microbiology and Immunology
Identifiers: 991014878275604721

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Collaboration types: Domestic collaboration; International collaboration
Web of Science research areas: Biochemical Research Methods

Efficient and specific removal of albumin from human serum samples

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Abstract

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UN Sustainable Development Goals (SDGs)

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