Journal article
Electrochemical detection of rapid DA release kinetics during hypoxia in perfused-superfused cat CB
Journal of applied physiology (1985), v 78(3), pp 830-837
01 Mar 1995
PMID: 7775326
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The hypothesis that hypoxic excitation is coupled to dopamine (DA) secretion was tested in perfused-superfused cat carotid bodies (CB). DA was electrochemically detected by an amperometric method (constant applied potential +150 mV) with Nafion polymer-coated recessed gold microsensors (tip diameter 3–8 microns) in 10 cat CBs. Neural discharge (ND) from the whole sinus nerve was measured simultaneously with DA changes during interruption of perfusate flow and during hypoxic perfusion (5% O2). A computer-controlled instrument using a chronoamperometric technique (+550-mV pulses) with a Nafion-coated carbon fiber microelectrode (tip diameter 35 microns) was used to detect DA changes in two CBs during similar hypoxic stimuli. Rapid DA release kinetics were measured during flow interruption with an initial rate of 1.05 +/- 0.15 (SE) microM/s within the first 10–15 s. At most measurement sites, the increase in DA preceded the rise in ND. After the initial increase, DA release slowed to 0.16 +/- 0.02 microM/s, reaching a maximum DA concentration of 20.7 +/- 2.6 microM above baseline after 90 s of flow interruption. Nicotine (10-micrograms bolus) caused a large increase in ND without a proportional increase in DA release. Spatially detailed time-resolved electrochemical measurements were able to discriminate between DA release during hypoxia and chemoexcitatory responses that do not involve DA release.
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Details
- Title
- Electrochemical detection of rapid DA release kinetics during hypoxia in perfused-superfused cat CB
- Creators
- D. G. Buerk - University of PennsylvaniaS. Lahiri - Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia 19104, USAD. Chugh - Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia 19104, USAA. Mokashi - Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA
- Publication Details
- Journal of applied physiology (1985), v 78(3), pp 830-837
- Publisher
- American Physiological Society (APS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:A1995QP82600013
- Scopus ID
- 2-s2.0-0028933479
- Other Identifier
- 991019231730304721
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- Web of Science research areas
- Physiology
- Sport Sciences