Journal article
Electrospun gelatin/hyaluronic acid nanofibers as a platform for uric acid delivery to neural tissue
Biotechnology progress, v 41(2), e3517
10 Nov 2024
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Abstract Uric acid (UA) is an antioxidant that has been reported to be a neuroprotective compound for injuries and diseases, and specifically, diseases of the central nervous system. However, uric acid is highly insoluble in aqueous solutions, and high levels in the serum lead to gout, which limits its use in humans. Here, we develop a novel drug delivery platform that will release uric acid in a sustained manner for application to neural tissue. We demonstrate that one‐step incorporation of UA into an electrospun gelatin/hyaluronic acid nanofiber mat results in controlled release of UA in culture medium. Taking a unique approach, we made solutions of 12% gelatin and 1% hyaluronic acid in a formic acid solvent and added UA for production of nanofiber mats. We then dehydrothermally crosslinked the mats and tested for release of UA into physiological cell culture medium. To test whether the mats have any detrimental effects on healthy nervous system tissue, we cultured spinal cord explants on the mats extended and assessed extensions from the explants. We observed that comparable numbers and lengths of dendrites are extended from the spinal cord tissue, regardless of the amount UA content in the mats. Our results suggest that electrospun gelatin/hyaluronic acid nanofibers can be used as a platform for sustained uric acid delivery to neural tissue without detrimental effects.
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Details
- Title
- Electrospun gelatin/hyaluronic acid nanofibers as a platform for uric acid delivery to neural tissue
- Creators
- Reva M. Street - Drexel UniversityFrank H. Kung - Rutgers, The State University of New JerseyLaura T. Beringer - Drexel UniversityDaniel B. Amchin - Drexel UniversityBonnie L. Firestein - Rutgers, The State University of New JerseyCaroline L. Schauer (Corresponding Author) - Drexel University
- Publication Details
- Biotechnology progress, v 41(2), e3517
- Publisher
- Wiley
- Number of pages
- 8
- Grant note
- New Jersey Commission on Spinal Cord Research: CSCR14IRG005 New Jersey Commission on Spinal Cord Research (NJCSCR): P200A120253 Graduate Assistance in Areas of National Need (GAANN) FellowshipDrexel University Freshman Design Fellowship
This work is funded in part by Funding for research provided by the New Jersey Commission on Spinal Cord Research (NJCSCR) grant CSCR14IRG005 (to B.L.F.). Researcher support for R.M.S. was provided by the Graduate Assistance in Areas of National Need (GAANN) Fellowship (Award # P200A120253) and the Drexel University Freshman Design Fellowship. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Materials Science and Engineering; College of Engineering
- Web of Science ID
- WOS:001354108100001
- Scopus ID
- 2-s2.0-105002569255
- Other Identifier
- 991021961115504721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Food Science & Technology