Journal article
Elucidating nanoscale mechanical properties of diabetic human adipose tissue using atomic force microscopy
Scientific reports, v 10(1), 20423
24 Nov 2020
PMID: 33235234
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Obesity-related type 2 diabetes (DM) is a major public health concern. Adipose tissue metabolic dysfunction, including fibrosis, plays a central role in DM pathogenesis. Obesity is associated with changes in adipose tissue extracellular matrix (ECM), but the impact of these changes on adipose tissue mechanics and their role in metabolic disease is poorly defined. This study utilized atomic force microscopy (AFM) to quantify difference in elasticity between human DM and non-diabetic (NDM) visceral adipose tissue. The mean elastic modulus of DM adipose tissue was twice that of NDM adipose tissue (11.50 kPa vs. 4.48 kPa) to a 95% confidence level, with significant variability in elasticity of DM compared to NDM adipose tissue. Histologic and chemical measures of fibrosis revealed increased hydroxyproline content in DM adipose tissue, but no difference in Sirius Red staining between DM and NDM tissues. These findings support the hypothesis that fibrosis, evidenced by increased elastic modulus, is enhanced in DM adipose tissue, and suggest that measures of tissue mechanics may better resolve disease-specific differences in adipose tissue fibrosis compared with histologic measures. These data demonstrate the power of AFM nanoindentation to probe tissue mechanics, and delineate the impact of metabolic disease on the mechanical properties of adipose tissue.
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Details
- Title
- Elucidating nanoscale mechanical properties of diabetic human adipose tissue using atomic force microscopy
- Creators
- J. K. Wenderott - University of MichiganCarmen G. Flesher - A. Alfred Taubman Health Care CenterNicki A. Baker - A. Alfred Taubman Health Care CenterChristopher K. Neeley - A. Alfred Taubman Health Care CenterOliver A. Varban - A. Alfred Taubman Health Care CenterCarey N. Lumeng - University of MichiganLutfiyya N. Muhammad - Northwestern UniversityChen Yeh - Northwestern UniversityPeter F. Green - BioSurfacesRobert W. O'Rourke - University of Michigan
- Publication Details
- Scientific reports, v 10(1), 20423
- Publisher
- Nature Publishing Group
- Number of pages
- 9
- Grant note
- I01CX001811 / Veterans Affairs Merit Grant; US Department of Veterans Affairs R01DK115190; R01DK090262 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA DMR-1305749 / National Science Foundation (NSF) Division of Materials Research; National Science Foundation (NSF) DE-SC0000957 / Department of Energy, Energy Frontier Research Center; United States Department of Energy (DOE) P30DK089503 / National Institute of Diabetes and Digestive and Kidney Diseases; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Materials Science and Engineering
- Web of Science ID
- WOS:000596278900008
- Scopus ID
- 2-s2.0-85096546738
- Other Identifier
- 991022132152104721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Endocrinology & Metabolism