Journal article
Embryonic and early postnatal cranial bone volume and tissue mineral density values for C57BL/6J laboratory mice
DEVELOPMENTAL DYNAMICS, v 251(7), p1196
Jul 2022
PMID: 35092111
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Background Laboratory mice are routinely used in craniofacial research based on the relatively close genetic relationship and conservation of developmental pathways between humans and mice. Since genetic perturbations and disease states may have localized effects, data from individual cranial bones are valuable for the interpretation of experimental assays. We employ high-resolution microcomputed tomography to characterize cranial bones of C57BL/6J mice at embryonic day (E) 15.5 and E17.5, day of birth (P0), and postnatal day 7 (P7) and provide estimates of individual bone volume and tissue mineral density (TMD). Results Average volume and TMD values are reported for individual bones. Significant differences in volume and TMD during embryonic ages likely reflect early mineralization of cranial neural crest-derived and intramembranously forming bones. Although bones of the face and vault had higher TMD values during embryonic ages, bones of the braincase floor had significantly higher TMD values by P7. Conclusions These ontogenetic data on cranial bone volume and TMD serve as a reference standard for future studies using mice bred on a C57BL/6J genetic background. Our findings also highlight the importance of differentiating control data from mice that are presented as unaffected littermates, particularly when carrying a single copy of a cre-recombinase gene.
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Details
- Title
- Embryonic and early postnatal cranial bone volume and tissue mineral density values for C57BL/6J laboratory mice
- Publication Details
- DEVELOPMENTAL DYNAMICS, v 251(7), p1196
- Publisher
- WILEY; HOBOKEN
- Grant note
- Eunice Kennedy Shriver National Institute of Child Health and Human Development, Grant/Award Number: P01HD078233; National Institute of Dental and Craniofacial Research, Grant/Award Number: R01DE027677; National Science Foundation, Grant/Award Number: BCS 1731909
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Drexel University
- Web of Science ID
- WOS:000751853500001
- Scopus ID
- 2-s2.0-85124462844
- Other Identifier
- 991021861290804721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Anatomy & Morphology
- Developmental Biology