Journal article
Endogenous and Expressed Angiotensin II Receptors on Xenopus Oocytes
Journal of neurochemistry, v 56(4), pp 1307-1311
Apr 1991
PMID: 2002343
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
: Intact Xenopus oocytes contain a homogeneous population of binding sites for the angiotensin II (Ang II) receptor antagonist 125I‐[Sarc1,Ile8]‐Ang II (125I‐SARILE). Binding of 125I‐SARILE to intact oocytes was saturable and of high affinity with an apparent KD of 0.7 nM and maximal density of 0.12 fmol/oocyte. Binding of 125I‐SARILE to oocytes also was specific for Ang II‐related peptides with a rank order potency of: [Sarc1]‐Ang II > Ang II > Ang III Ang I. However, these endogenous binding sites were present only in follicle‐enclosed oocytes and within the follicular layer itself. On the other hand, injection of poly(A)+ RNA isolated from murine N1E‐115 neuroblastoma cells into oocytes resulted in the appearance of 125I‐SARILE binding sites even in defolliculated oocytes. These expressed receptors exhibited pharmacological properties similar to those endogenously present in the follicular layer, although their levels were much less. Collectively, these results suggest that endogenous Ang II receptors are present on Xenopus oocyte follicle cells, whereas Ang II receptors expressed from exogenous N1E‐115 RNA are found on the oocytes themselves. In addition, the high density of Ang II receptors on the follicle cells emphasizes the necessity for care in using Xenopus oocytes for the expression of receptors encoded by exogenous RNAs.
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Details
- Title
- Endogenous and Expressed Angiotensin II Receptors on Xenopus Oocytes
- Creators
- Steven J. Fluharty - University of PennsylvaniaLawrence P. Reagan - University of PennsylvaniaMichael M. White - University of Pennsylvania
- Publication Details
- Journal of neurochemistry, v 56(4), pp 1307-1311
- Publisher
- Blackwell Publishing Ltd
- Number of pages
- 5
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:A1991FB65600029
- Scopus ID
- 2-s2.0-0026063839
- Other Identifier
- 991020837830204721
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Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Neurosciences