Journal article
Endogenous prostaglandins modulate chloride secretion by prairie dog gallbladder
The Journal of laboratory and clinical medicine, v 135(1)
2000
PMID: 10638698
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In addition to concentrating bile, the gallbladder secretes chloride (Cl
-) and mucus into its lumen. We recently observed that gallbladder Cl
- secretion is increased in prairie dogs during the formation of cholesterol crystals, a period of altered mucosal prostaglandin synthesis. Pathologic Cl
- secretion is characteristic of other epithelial disorders such as cystic fibrosis and hypercalciuric nephrolithiasis and may be important in gallstone pathogenesis. We hypothesized that concentrations of endogenous prostaglandin E
2 (PGE
2) found during experimental gallstone formation may mediate increased Cl
- secretion by prairie dog gallbladder. Prairie dog gallbladders were harvested by cholecystectomy and mounted in Ussing chambers. Unidirectional transepithelial Cl
-, Na
+, and H
2O fluxes were measured before and after inhibition of endogenous prostaglandin synthesis with 10 μmol/L indomethacin. Gallbladders were then exposed to increasing concentrations of PGE
2 to a maximal dose of 1 μmol/L, as found in animals with gallstones. Standard electrophysiologic parameters were recorded simultaneously. Indomethacin increased mucosal resistance and stimulated gallbladder Na
+ and Cl
- absorption. These effects were rapidly reversed by PGE
2. PGE
2 promoted Cl
- secretion and decreased mucosal Na
+ absorption at concentrations found in the gallbladder bile of animals with gallstones. Endogenous prostaglandin metabolism modulates gallbladder Cl
- secretion and may promote changes in Cl
- transport associated with cholelithiasis. (J Lab Clin Med 2000;135:82-88)
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Details
- Title
- Endogenous prostaglandins modulate chloride secretion by prairie dog gallbladder
- Creators
- A.James Moser - Pittsburgh and Philadelphia, PennsylvaniaMohammad Z. Abedin - Pittsburgh and Philadelphia, PennsylvaniaKenneth E. Morgenstern - Pittsburgh and Philadelphia, PennsylvaniaZahidur R. Abedin - Pittsburgh and Philadelphia, PennsylvaniaJoel J. Roslyn - Pittsburgh and Philadelphia, Pennsylvania
- Publication Details
- The Journal of laboratory and clinical medicine, v 135(1)
- Publisher
- Mosby, Inc
- Resource Type
- Journal article
- Language
- English
- Web of Science ID
- WOS:000086240100012
- Scopus ID
- 2-s2.0-0033986674
- Other Identifier
- 991019318946604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Medical Laboratory Technology
- Medicine, Research & Experimental