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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Engineering Approaches for the Detection and Control of Orthopaedic Biofilm Infections
Clinical orthopaedics and related research, (437), pp 59-66
Aug 2005
PMID: 16056027
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Artificial joints are subject to chronic infections associated with bacterial biofilms, which only can be eradicated by the traumatic removal of the implant followed by sustained intravenous antibiotic therapy. We have adopted an engineering approach to develop electrical–current-based approaches to bacterial eradication and microelectromechanical systems that could be embedded within the implanted joint to detect the presence of bacteria and to provide in situ treatment of the infection before a biofilm can form. In the former case we will examine the combined bactericidal effects of direct and indirect electrical fields in combination with antibiotic therapy. In the latter case, bacterial detection will occur by developing a microelectromechanical–systems-based biosensor that can “eavesdrop” on bacterial quorum–sensing-based communication systems. Treatment will be effected by the release of a cocktail of pharmaceutical reagents contained within integral reservoirs associated with the implant, including a molecular jamming signal that competitively binds to the bacteria’s quorum sensing receptors (which will “blind” the bacteria, preventing the production of toxins) and multiple high dose antibiotics to eradicate the planktonic bacteria. This approach is designed to take advantage of the relatively high susceptibility to antibiotics that planktonic bacteria display compared with biofilm envirovars. Here we report the development of a generic microelectromechanical systems biosensor that measures changes in internal viscosity in a base fluid triggered by a change in the external environment.
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Details
- Title
- Engineering Approaches for the Detection and Control of Orthopaedic Biofilm Infections
- Creators
- Garth D Ehrlich - From the Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA; thePaul Stoodley - From the Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA; theSandeep Kathju - From the Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA; theYongjun Zhao - Department of Mechanical Engineering, Carnegie Mellon University, Pittsburgh, PA; theBruce R McLeod - Center for Biofilm Engineering Montana State University, Bozeman, MT; theNaomi Balaban - Department of Biomedical Sciences, Tufts University School of Veterinary Medicine, North Grafton, MA; theFen Ze Hu - From the Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA; theNicholas G Sotereanos - From the Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA; theJ. William Costerton - Center for Biofilm Engineering Montana State University, Bozeman, MT; thePhilip S Stewart - Center for Biofilm Engineering Montana State University, Bozeman, MT; theJ. Christopher Post - From the Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA; theQiao Lin - Department of Mechanical Engineering, Carnegie Mellon University, Pittsburgh, PA; the
- Publication Details
- Clinical orthopaedics and related research, (437), pp 59-66
- Publisher
- Lippincott
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000231072500011
- Scopus ID
- 2-s2.0-23244442259
- Other Identifier
- 991014877940204721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Orthopedics
- Surgery