Journal article
Engineering Highly Vascularized Bone Tissues by 3D Bioprinting of Granular Prevascularized Spheroids
ACS applied materials & interfaces, v 15(37), pp 43492-43502
20 Sep 2023
PMID: 37691550
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The convergence of 3D bioprinting with powerful manufacturing capability and cellular self-organization that can reproduce intricate tissue microarchitecture and function is a promising direction toward building functional tissues and has yet to be demonstrated. Here, we develop a granular aggregate-prevascularized (GAP) bioink for engineering highly vascularized bone tissues by capitalizing on the condensate-mimicking, self-organization, and angiogenic properties of prevascularized mesenchymal spheroids. The GAP bioink utilizes prevascularized aggregates as building blocks, which are embedded densely in extracellular matrices conducive to spontaneous self-organization. We printed various complex structures with high cell density (∼1.5 × 108 cells/cm3), viability (∼80%), and shape fidelity using GAP bioink. After printing, the prevascularized mesenchymal spheroids developed an interconnected vascular network through angiogenic sprouting. We printed highly vascularized bone tissues using GAP bioink and found that prevascularized spheroids were more conducive to osteogenesis and angiogenesis. We envision that the design of the GAP bioink could be further integrated with human-induced pluripotent stem cell-derived organoids, which opens new avenues to create patient-specific vascularized tissues for therapeutic applications..
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Details
- Title
- Engineering Highly Vascularized Bone Tissues by 3D Bioprinting of Granular Prevascularized Spheroids
- Creators
- Yongcong Fang - Biomanufacturing and Engineering Living Systems” Innovation International Talents Base (111 Base)Mengke Ji - Tsinghua UniversityBingyan Wu - Tsinghua UniversityXinxin Xu - Chinese PLA General HospitalGe Wang - Tsinghua UniversityYanmei Zhang - Biomanufacturing and Engineering Living Systems” Innovation International Talents Base (111 Base)Yingkai Xia - Tsinghua UniversityZhe Li - Tsinghua UniversityTing Zhang - Tsinghua UniversityWei Sun - Drexel University, Mechanical Engineering and MechanicsZhuo Xiong - Biomanufacturing and Engineering Living Systems” Innovation International Talents Base (111 Base)
- Publication Details
- ACS applied materials & interfaces, v 15(37), pp 43492-43502
- Publisher
- American Chemical Society
- Number of pages
- 11
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:001065974000001
- Scopus ID
- 2-s2.0-85171900022
- Other Identifier
- 991021861180504721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Materials Science, Multidisciplinary
- Nanoscience & Nanotechnology