Journal article
Engineering-derived approaches for iPSC preparation, expansion, differentiation and applications
Biofabrication, v 9(3), pp 032001/1-032001/19
31 Jul 2017
PMID: 28759433
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Remarkable achievements have been made since induced pluripotent stem cells (iPSCs) were first introduced in 2006. Compared with non-pluripotent stem cells, iPSC research faces several additional complexities, such as the choice of extracellular matrix proteins, growth and differentiation factors, as well as technical challenges related to self-renewal and directed differentiation. Overcoming these challenges requires the integration of knowledge and technologies from multiple fields including cell biology, biomaterial science, engineering, physics and medicine. Here, engineering-derived iPSC approaches are reviewed according to three aspects of iPSC studies: preparation, expansion, differentiation and applications. Engineering strategies, such as 3D systems establishment, cell-matrix mechanics and the regulation of biophysical and biochemical cues, together with engineering techniques, such as 3D scaffolds, cell microspheres and bioreactors, have been applied to iPSC studies and have generated insightful results and even mini-organs such as retinas, livers and intestines. Specific results are given to demonstrate how these approaches impact iPSC behavior, and related mechanisms are discussed. In addition, cell printing technologies are presented as an advanced engineering-derived approach since they have been applied in both iPSC studies and the construction of diverse tissues and organs. Further development and possible innovations of cell printing technologies are presented in terms of creating complex and functional iPSC-derived living tissues and organs.
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Details
- Title
- Engineering-derived approaches for iPSC preparation, expansion, differentiation and applications
- Creators
- Yang Li - Department of Mechanical Engineering, 111 'Biomanufacturing and Engineering Living Systems' Innovation International Talents Base, Tsinghua University, Beijing, People's Republic of China.Ling Li - Air Force Medical UniversityZhi-Nan Chen - Air Force Medical UniversityGe Gao - Ixcell Biotech Shanghai, Shanghai, People’s Republic of ChinaRui Yao - Tsinghua UniversityWei Sun - Drexel University
- Publication Details
- Biofabrication, v 9(3), pp 032001/1-032001/19
- Publisher
- IOP Publishing
- Number of pages
- 19
- Grant note
- 2014z21030 / Tsinghua University (https://doi.org/10.13039/501100004147) 31500818; 51235006 / National Natural Science Foundation of China (https://doi.org/10.13039/501100001809)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000406665000001
- Scopus ID
- 2-s2.0-85027883336
- Other Identifier
- 991019167660104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials