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Engraftment of peripheral blood mononuclear cells from patients with type 1 diabetes in NOD-scid HLA-A0201/γc -/-immunodeficient mice (93.5)
Journal article   Peer reviewed

Engraftment of peripheral blood mononuclear cells from patients with type 1 diabetes in NOD-scid HLA-A0201/γc -/-immunodeficient mice (93.5)

Fatima Larry, Ellen Young, Elizabeth Fudge, Anita Azam, Shipra Patel, Largay Joseph, Byrd Warren, John Buse, Ali Calikoglu and Jeffrey Frelinger
The Journal of immunology (1950), v 184(1_Supplement), pp 93-93.5
01 Apr 2010
DOI: https://doi.org/10.4049/jimmunol.184.Supp.93.5

Abstract

Abstract Type 1 diabetes (T1D) is an autoimmune disease characterized by insulitis followed by CD4+ and CD8+ T cell-mediated destruction of the insulin-producing pancreatic beta cells. Studies have demonstrated the presence of circulating islet-antigen specific CD4+ and CD8+ T cells during the clinical onset of disease. Over the past twenty years non-obese diabetic (NOD) mice have provided a useful tool for understanding human T1D. However, the study of human CD4+ and CD8+ T cell-mediated responses and the development of novel therapeutics for T1D requires an in vivo humanized animal model. In order to develop a model where we could investigate the function of human diabetogenic T cells in vivo we transferred peripheral blood mononuclear cells (PBMCs) from patients with T1D into NOD-scid HLA-A*0201/γc-/- transgenic immunodeficient mice. Following adoptive transfer we examined human T cell survival and homing. We isolated human CD4+ and CD8+ T cells from the pancreatic lymph node, spleen and islets of these mice for up to seven weeks. Our data suggest that this may be a useful humanized mouse model system for studying type 1 diabetes.

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