Journal article
Enhanced protein kinase C activity and translocation in bipolar affective disorder brains
Biological psychiatry (1969), v 40(7), pp 568-575
1996
PMID: 8886289
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Abstract
Protein kinase C (PKC) activity and its redistribution were determined in the frontal cortices of postmortem brains of bipolar affective disorder subjects and age-, sex-, and postmortem time-matched controls. Membrane and cytosolic PKC activity was determined by histone phosphorylation using [
32P]-adenosine triphosphate as substrate. Specific PKC isozyme levels were assessed by Western blot analysis using antipeptide antibodies. Brain membraneassociated PKC activity was higher in bipolar vs. control tissue. An examination of the specific PKC isozymes in cortical homogenates revealed that cytosolic α- and membrane-associated γ- and ζ PKC isozymes were elevated in cortices of bipolar affective disorder subjects, whereas cytosolic εPKC was found to be reduced. In control brain slices, incubation with 1
μmol/L phorbol 12-myristate 13-acetate (PMA) caused an increase in membrane PKC activity, whereas cytosolic enzyme activity was decreased. This redistribution of the enzyme by PMA was markedly potentiated in brain slices of bipolar subjects. The results suggest that PKC-mediated phosphorylation is increased in brains of subjects with bipolar affective illness.
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Details
- Title
- Enhanced protein kinase C activity and translocation in bipolar affective disorder brains
- Creators
- Hoau-Yan Wang - Drexel UniversityEitan Friedman - Drexel University
- Publication Details
- Biological psychiatry (1969), v 40(7), pp 568-575
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:A1996VJ92900002
- Scopus ID
- 2-s2.0-0030273260
- Other Identifier
- 991019168724104721
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Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Neurosciences
- Psychiatry