Journal article
Enhanced reseeding of decellularized rodent lungs with mouse embryonic stem cells
Biomaterials, v 35(10), pp 3252-3262
Mar 2014
PMID: 24439414
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Repopulation of decellularized lung scaffolds (DLS) is limited due to alterations in the repertoire and ratios of the residual extracellular matrix (ECM) proteins, characterized by e.g., the retention of type I collagen and loss of glycoproteins. We hypothesized that pre-treatment of decellularized matrices with defined ECM proteins, which match the repertoire of integrin receptors expressed by the cells to be seeded (e.g., embryonic stem cells) can increase the efficacy of the reseeding process. To test this hypothesis, we first determined the integrin receptors profile of mouse embryonic stem cells (mESCs). Mouse ESCs express α3, α5, α6, α9 and β1, but not α1, α2 and α4 integrin subunits, as established by Western blotting and adhesion to laminin and fibronectin, but not to collagens type I and IV. Reseeding of DLS with mESCs was inefficient (6.9 ± 0.5%), but was significantly enhanced (2.3 ± 0.1 fold) by pre-treating the scaffolds with media conditioned by A549 human lung adenocarcinoma cells, which we found to contain ∼5 μg/ml laminin. Furthermore, pre-treatment with A549-conditioned media resulted in a significantly more uniform distribution of the seeded mESCs throughout the engineered organ as compared to untreated DLS. Our study may advance whole lung engineering by stressing the importance of matching the integrin receptor repertoire of the seeded cells and the cell binding motifs of DLS.
Metrics
Details
- Title
- Enhanced reseeding of decellularized rodent lungs with mouse embryonic stem cells
- Creators
- Shimon Lecht - Department of Bioengineering, College of Engineering, Temple University, Philadelphia, PA 19122, USACollin T Stabler - Department of Bioengineering, College of Engineering, Temple University, Philadelphia, PA 19122, USAAlexis L Rylander - Department of Bioengineering, College of Engineering, Temple University, Philadelphia, PA 19122, USARachel Chiaverelli - Department of Bioengineering, College of Engineering, Temple University, Philadelphia, PA 19122, USAEdward S Schulman - Division of Pulmonary, Critical Care and Sleep Medicine, Drexel University College of Medicine, Philadelphia, PA 19102, USACezary Marcinkiewicz - Department of Bioengineering, College of Engineering, Temple University, Philadelphia, PA 19122, USAPeter I Lelkes - Department of Bioengineering, College of Engineering, Temple University, Philadelphia, PA 19122, USA. Electronic address: pilelkes@temple.edu
- Publication Details
- Biomaterials, v 35(10), pp 3252-3262
- Publisher
- Elsevier; Netherlands
- Grant note
- T34 GM087239 / NIGMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pulmonary, Critical Care, and Sleep (Medicine)
- Web of Science ID
- WOS:000332188400012
- Scopus ID
- 2-s2.0-84893682122
- Other Identifier
- 991014877809904721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials