Journal article
Enhancement of Microbiota in Healthy Macaques Results in Beneficial Modulation of Mucosal and Systemic Immune Function
The Journal of immunology (1950), v 196(5), pp 2401-2409
01 Mar 2016
PMID: 26826246
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Given the critical role of mucosal surfaces in susceptibility to infection, it is imperative that effective mucosal responses are induced when developing efficacious vaccines and prevention strategies for infection. Modulating the microbiota in the gastrointestinal (GI) tract through the use of probiotics (PBio) is a safe and well-tolerated approach to enhance mucosal and overall health. We assessed the longitudinal impact of daily treatment with the VSL#3 probiotic on cellular and humoral immunity and inflammation in healthy macaques. PBio therapy resulted in significantly increased frequencies of B cells expressing IgA in the colon and lymph node (LN), likely because of significantly increased LN T follicular helper cell frequencies and LN follicles. Increased frequencies of IL-23(+) APCs in the colon were found post-PBio treatment, which correlated with LN T follicular helper cells. Finally, VSL#3 significantly downmodulated the response of TLR2-, TLR3-, TLR4-, and TLR9-expressing HEK293 cells to stimulation with Pam3CSK4, polyinosinic-polycytidylic acid, LPS, and ODN2006, respectively. These data provide a mechanism for the beneficial impact of PBio on mucosal health and implicates the use of PBio therapy in the context of vaccination or preventative approaches to enhance protection from mucosal infection by improving immune defenses at the mucosal portal of entry.
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Details
- Title
- Enhancement of Microbiota in Healthy Macaques Results in Beneficial Modulation of Mucosal and Systemic Immune Function
- Creators
- Jennifer A Manuzak - University of WashingtonTiffany Hensley-McBain - University of WashingtonAlexander S Zevin - University of WashingtonCharlene Miller - University of WashingtonRafael Cubas - GenentechBrian Agricola - Washington National Primate Research Center, Seattle, WA 98121;Jill Gile - University of WashingtonLaura Richert-Spuhler - University of WashingtonGabriela Patilea - University of WashingtonJacob D Estes - Leidos (United States)Stanley Langevin - University of WashingtonR Keith ReevesElias K Haddad - Drexel UniversityNichole R Klatt - University of Washington
- Publication Details
- The Journal of immunology (1950), v 196(5), pp 2401-2409
- Publisher
- American Association of Immunologists (AAI)
- Grant note
- P51 OD011107 / NIH HHS K22 AI098440 / NIAID NIH HHS 1R21AI120795-01 / NIAID NIH HHS R01 AI120712 / NIAID NIH HHS 1R01AI120712-01 / NIAID NIH HHS R21 AI120795 / NIAID NIH HHS P51 OD010425 / NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Medicine; Infectious Diseases (and HIV Medicine); Drexel University
- Web of Science ID
- WOS:000372336300042
- Scopus ID
- 2-s2.0-84962026552
- Other Identifier
- 991020100208104721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Immunology