Journal article
Enhancement of Tight Junctional Barrier Function by Micronutrients: Compound-Specific Effects on Permeability and Claudin Composition
PloS one, v 8(11), pp e78775-e78775
13 Nov 2013
PMID: 24236048
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Amid an increasing number of reports in the literature concerning epithelial barrier enhancement by various nutrient compounds, there has never been a study performing side-by-side comparisons of these agents in a single epithelial model. We compare five nutrient compounds (previously reported in various epithelial models to enhance barrier function) regarding their ability to increase transepithelial electrical resistance (R-t) and decrease transepithelial mannitol permeability (J(m)) across LLC-PK1 renal epithelial cell layers. The effects of these nutrients on the abundance of various tight junctional proteins are also compared. In the overall group of nutrients tested - zinc, indole, quercetin, butyrate and nicotine - only nicotine failed to improve barrier function by either parameter. Nicotine also was without effect on tight junctional proteins. Quercetin simultaneously increased R-t and decreased J(m). Zinc, butyrate and indole only exhibited statistically significant enhancement of R-t. Each of these four effective nutrient compounds had unique patterns of effects on the panel of tight junctional proteins studied. No two compounds produced the same pattern of effects. This unique pattern of effects on tight junctional complex composition by each compound establishes the chance for additive or even synergistic improvement of barrier function by combinations of compounds. A synergistic effect of the combination of quercetin and zinc on R-t is shown.
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Details
- Title
- Enhancement of Tight Junctional Barrier Function by Micronutrients: Compound-Specific Effects on Permeability and Claudin Composition
- Creators
- Joanna Mercado - Lankenau Institute for Medical ResearchMary Carmen Valenzano - Lankenau Institute for Medical ResearchCameron Jeffers - Drexel UniversityJason Sedlak - Drexel UniversityMarina K. Cugliari - Drexel UniversityEleni Papanikolaou - Drexel UniversityJacob Clouse - Drexel UniversityJingya Miao - Drexel UniversityNina E. Wertan - Lankenau Institute for Medical ResearchJames M. Mullin - Lankenau Institute for Medical ResearchChristos D Katsetos
- Publication Details
- PloS one, v 8(11), pp e78775-e78775
- Publisher
- Public Library Science
- Number of pages
- 8
- Grant note
- Sharpe Strumia Foundation
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000327254700045
- Scopus ID
- 2-s2.0-84893584653
- Other Identifier
- 991019353725904721
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Nutrition & Dietetics