Journal article
EphrinBs Regulate D-Serine Synthesis and Release in Astrocytes
The Journal of neuroscience, v 30(47), pp 16015-16024
24 Nov 2010
PMID: 21106840
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
There is growing evidence that astrocytes play critical roles in neuron-glial interactions at the synapse. Astrocytes are believed to regulate presynaptic and postsynaptic structures and functions, in part, by the release of gliotransmitters such as glutamate, ATP, and D-serine; however, little is known of how neurons and astrocytes communicate to regulate these processes. Here, we investigated a family of transmembrane proteins called ephrinBs and Eph receptors that are expressed in the synapse and are known to regulate synaptic transmission and plasticity. In addition to their presence on CA1 hippocampal neurons, we determined that ephrins and Eph receptors are also expressed on hippocampal astrocytes. Stimulation of hippocampal astrocytes with soluble ephrinB3, known to be expressed on CA1 postsynaptic dendrites, enhanced D-serine synthesis and release in culture. Conversely, ephrinB3 had no effect on D-serine release from astrocytes deficient in EphB3 and EphA4, which are the primary receptors for ephrinB3. Eph receptors mediate this response through interactions with PICK1 (protein interacting with C-kinase) and by dephosphorylating protein kinase C alpha to activate the conversion of L-serine to D-serine by serine racemase. These findings are supported in vivo, where reduced D-serine levels and synaptic transmissions are observed in the absence of EphB3 and EphA4. These data support a role for ephrins and Eph receptors in regulating astrocyte gliotransmitters, which may have important implications on synaptic transmission and plasticity.
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Details
- Title
- EphrinBs Regulate D-Serine Synthesis and Release in Astrocytes
- Creators
- Zhiye Zhuang - University of MiamiBing Yang - University of MiamiMichelle H. Theus - University of MiamiJustin T. Sick - University of MiamiJohn R. Bethea - Neurological SurgeryThomas J. Sick - University of MiamiDaniel J. Liebl - Neurological Surgery
- Publication Details
- The Journal of neuroscience, v 30(47), pp 16015-16024
- Publisher
- Soc Neuroscience
- Number of pages
- 10
- Grant note
- NS049545; NS30291 / National Institutes of Health-National Institute of Neurological Disorders and Stroke; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) R01NS049545 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) Miami Project to Cure Paralysis
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology; College of Arts and Sciences; Drexel University
- Web of Science ID
- WOS:000284580100031
- Scopus ID
- 2-s2.0-78649414612
- Other Identifier
- 991020099925204721
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- Web of Science research areas
- Neurosciences