Files and links (1)
Published, Version of Record (VoR)CC BY-NC-ND V4.0, Open
Journal article
Epidermal Growth Factor Receptor Inhibition Reverses Cellular and Transcriptomic Alterations Induced by Hypoxia in the Neonatal Piglet Brain
iScience, Vol.23(12), 101766
18 Dec 2020
PMID: 33294779
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Acute hypoxia (HX) causes extensive cellular damage in the developing human cerebral cortex. We found increased expression of activated-EGFR in affected cortical areas of neonates with HX and investigated its functional role in the piglet, which displays a highly evolved, gyrencephalic brain, with a human-like maturation pattern. In the piglet, HX-induced activation of EGFR and Ca2+/calmodulin kinase IV (CaMKIV) caused cell death and pathological alterations in neurons and glia. EGFR blockade inhibited CaMKIV activation, attenuated neuronal loss, increased oligodendrocyte proliferation, and reversed HX-induced astrogliosis. We performed for the first time high-throughput transcriptomic analysis of the piglet cortex to define molecular responses to HX and to uncover genes specifically involved in EGFR signaling in piglet and human brain injury. Our results indicate that specific molecular responses modulated by EGFR may be targeted as a therapeutic strategy for HX injury in the neonatal brain.
[Display omitted]
•EGFR mediates the effects of neonatal hypoxia in piglet and human cerebral cortex•EGFR inhibition reverses pathological changes induced by hypoxia in piglet cortex•EGFR blockage prevents hypoxia-induced transcriptomic changes in piglet cortex•EGFR-associated pathways are therapeutic targets in neonatal hypoxic injury
Porcine Molecular Biology; Developmental Neuroscience; Transcriptomics
Metrics
7 Record Views
Details
- Title
- Epidermal Growth Factor Receptor Inhibition Reverses Cellular and Transcriptomic Alterations Induced by Hypoxia in the Neonatal Piglet Brain
- Creators
- Panagiotis Kratimenos - George Washington UniversityEvan Z. Goldstein - George Washington UniversityIoannis Koutroulis - George Washington UniversitySusan Knoblach - George Washington UniversityBeata Jablonska - George Washington UniversityPayal Banerjee - Children’s National Health SystemShadi N. Malaeb - Drexel UniversitySurajit Bhattacharya - Children’s National Health SystemM. Isabel Almira-Suarez - George Washington UniversityVittorio Gallo - George Washington UniversityMaria Delivoria-Papadopoulos - Drexel University
- Publication Details
- iScience, Vol.23(12), 101766
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics
- Identifiers
- 991019168009804721
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences