Journal article
Epigenetic Regulation of Condensin-Mediated Genome Organization during the Cell Cycle and upon DNA Damage through Histone H3 Lysine 56 Acetylation
Molecular cell, v 48(4), pp 532-546
30 Nov 2012
PMID: 23084836
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Complex genome organizations participate in various nuclear processes including transcription, DNA replication, and repair. However, the mechanisms that generate and regulate these functional genome structures remain largely unknown. Here, we describe how the Ku heterodimer complex, which functions in nonhomologous end joining, mediates clustering of long terminal repeat retrotransposons at centromeres in fission yeast. We demonstrate that the CENP-B subunit, Abp1, functions as a recruiter of the Ku complex, which in turn loads the genome-organizing machinery condensin to retrotransposons. Intriguingly, histone H3 lysine 56 (H3K56) acetylation, which functions in DNA replication and repair, interferes with Ku localization at retrotransposons without disrupting Abp1 localization and, as a consequence, dissociates condensin from retrotransposons. This dissociation releases condensin-mediated genomic associations during S phase and upon DNA damage. ATR (ATM- and Rad3-related) kinase mediates the DNA damage response of condensin-mediated genome organization. Our study describes a function of H3K56 acetylation that neutralizes condensin-mediated genome organization.
► Retrotransposon clustering is mediated by Ku, condensin, and CENP-B ► H3K56Ac regulates localization of Ku and condensin at retrotransposons ► Retrotransposon clustering is released during S phase and upon DNA damage ► ATR mediates disassembly of retrotransposon clustering upon DNA damage
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Details
- Title
- Epigenetic Regulation of Condensin-Mediated Genome Organization during the Cell Cycle and upon DNA Damage through Histone H3 Lysine 56 Acetylation
- Creators
- Atsunari Tanaka - Department of Gene Expression and Regulation, The Wistar Institute, Philadelphia, PA 19104, USAHideki Tanizawa - Department of Gene Expression and Regulation, The Wistar Institute, Philadelphia, PA 19104, USASira Sriswasdi - Department of Molecular and Cellular Oncogenesis, The Wistar Institute, Philadelphia, PA 19104, USAOsamu Iwasaki - Department of Gene Expression and Regulation, The Wistar Institute, Philadelphia, PA 19104, USAAtreyi G Chatterjee - Section on Eukaryotic Transposable Elements, Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USADavid W Speicher - Department of Molecular and Cellular Oncogenesis, The Wistar Institute, Philadelphia, PA 19104, USAHenry L Levin - Section on Eukaryotic Transposable Elements, Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USAEishi Noguchi - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102, USAKen-ichi Noma - Department of Gene Expression and Regulation, The Wistar Institute, Philadelphia, PA 19104, USA
- Publication Details
- Molecular cell, v 48(4), pp 532-546
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000311919500007
- Scopus ID
- 2-s2.0-84870390011
- Other Identifier
- 991014878023504721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology