Journal article
Epithelial polarity requires septin coupling of vesicle transport to polyglutamylated microtubules
The Journal of cell biology, v 180(2), pp 295-303
28 Jan 2008
PMID: 18209106
Abstract
In epithelial cells, polarized growth and maintenance of apical and basolateral plasma membrane domains depend on protein sorting from the trans-Golgi network (TGN) and vesicle delivery to the plasma membrane. Septins are filamentous GTPases required for polarized membrane growth in budding yeast, but whether they function in epithelial polarity is unknown. Here, we show that in epithelial cells septin 2 (SEPT2) fibers colocalize with a subset of microtubule tracks composed of polyglutamylated (polyGlu) tubulin, and that vesicles containing apical or basolateral proteins exit the TGN along these SEPT2/polyGlu microtubule tracks. Tubulin-associated SEPT2 facilitates vesicle transport by maintaining polyGlu microtubule tracks and impeding tubulin binding of microtubule-associated protein 4 (MAP4). Significantly, this regulatory step is required for polarized, columnar-shaped epithelia biogenesis; upon SEPT2 depletion, cells become short and fibroblast-shaped due to intracellular accumulation of apical and basolateral membrane proteins, and loss of vertically oriented polyGlu microtubules. We suggest that septin coupling of the microtubule cytoskeleton to post-Golgi vesicle transport is required for the morphogenesis of polarized epithelia.
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Details
- Title
- Epithelial polarity requires septin coupling of vesicle transport to polyglutamylated microtubules
- Creators
- Elias T Spiliotis - Departments of Biology, and Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305Stephen J Hunt - Departments of Biology, and Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305Qicong Hu - Departments of Biology, and Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305Makoto Kinoshita - Biochemistry and Cell Biology Unit, HMRO, Kyoto University, Kyoto 606-8501, JapanW. James Nelson - Departments of Biology, and Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305
- Publication Details
- The Journal of cell biology, v 180(2), pp 295-303
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000252746900008
- Scopus ID
- 2-s2.0-38749147682
- Other Identifier
- 991014878040604721
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- Collaboration types
- International collaboration
- Web of Science research areas
- Cell Biology