Journal article
Escherichia coli 83972 Expressing a P fimbriae Oligosaccharide Receptor Mimic Impairs Adhesion of Uropathogenic E-coli
The Journal of infectious diseases, v 206(8), pp 1242-1249
15 Oct 2012
PMID: 22872729
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) are a significant health concern, exacerbated by the rapid emergence of multidrug resistant strains refractory to antibiotic treatment. P fimbriae are strongly associated with upper urinary tract colonization due to specific binding to alpha-D-galactopyranosyl-(1-4)-beta-D-galactopyranoside receptors in the kidneys. Thus, inhibiting P-fimbrial adhesion may reduce the incidence of UPEC-mediated UTI. E. coli 83972 is an asymptomatic bacteriuria isolate successfully used as a prophylactic agent to prevent UTI in human studies. We constructed a recombinant E. coli 83972 strain displaying a surface-located oligosaccharide P fimbriae receptor mimic that bound to P-fimbriated E. coli producing any of the 3 PapG adhesin variants. The recombinant strain, E. coli 83972::lgtCE, impaired P fimbriae-mediated adhesion to human erythrocytes and kidney epithelial cells. Additionally, E. coli 83972::lgtCE impaired urine colonization by UPEC in a mouse UTI model, demonstrating its potential as a prophylactic agent to prevent UTI.
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Details
- Title
- Escherichia coli 83972 Expressing a P fimbriae Oligosaccharide Receptor Mimic Impairs Adhesion of Uropathogenic E-coli
- Creators
- Rebecca E. Watts - The University of QueenslandChee K. Tan - Griffith UniversityGlen C. Ulett - Griffith UniversityAlison J. Carey - Griffith UniversityMakrina Totsika - The University of QueenslandAdi Idris - The University of QueenslandAdrienne W. Paton - The University of AdelaideRenato Morona - The University of AdelaideJames C. Paton - The University of AdelaideMark A. Schembri - The University of Queensland
- Publication Details
- The Journal of infectious diseases, v 206(8), pp 1242-1249
- Publisher
- Oxford Univ Press
- Number of pages
- 8
- Grant note
- FT100100662; FT110101048 / ARC; Australian Research Council 569676; 631654 / Australian National Health and Medical Research Council; National Health and Medical Research Council (NHMRC) of Australia
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics; College of Medicine; Drexel University
- Web of Science ID
- WOS:000309463200012
- Scopus ID
- 2-s2.0-84866953041
- Other Identifier
- 991020100202904721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Immunology
- Infectious Diseases
- Microbiology