Journal article
Escherichia coli Inactivation by UVC-Irradiated C-60: Kinetics and Mechanisms
Environmental science & technology, v 45(22), pp 9627-9633
15 Nov 2011
PMID: 21999435
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Motivated by recent studies that documented changes in fullerene toxicity after chemical transformation, C-60 aggregates (nC(60)) were subject to UVC irradiation at monochromatic 254 nm and subsequently evaluated for antibacterial and bactericidal properties against Escherichia coli. The nC(60) treated with UVC irradiation, referred to herein as UVC-irradiated C-60, did not directly inhibit bacterial growth at concentrations up to 20 mg/L. In the presence of UVA and visible light, however, UVC-irradiated C-60 rapidly inactivated E. coli, suggesting that photochemical production of reactive oxygen species (ROS) was involved. The use of ROS scavengers and probes determined that hydroxyl radicals were the primary ROS responsible for the E. coli inactivation. Results from protein release, lipid peroxidation, cell permeability, and intracellular enzyme assays suggest that the inactivation mechanism involves UVC-irradiated diffusing through E. coli cell membrane and producing hydroxyl radicals within the cell. Further study on water-soluble C-60 derivatives and possible transformative processes is, therefore, recommended based on the environmental implications of results presented herein that nC(60) exposed to UVC irradiation is more toxic than parent nC(60).
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Details
- Title
- Escherichia coli Inactivation by UVC-Irradiated C-60: Kinetics and Mechanisms
- Creators
- Min Cho - Georgia Institute of TechnologySamuel D. Snow - Georgia Institute of TechnologyJoseph B. Hughes - Georgia Institute of TechnologyJae-Hong Kim - Georgia Institute of Technology
- Publication Details
- Environmental science & technology, v 45(22), pp 9627-9633
- Publisher
- Amer Chemical Soc
- Number of pages
- 7
- Grant note
- NRF-2011-35B-D00020 / Korean National Research Foundation (Korean Ministry of Education, Science and Technology); Ministry of Education, Science & Technology (MEST), Republic of Korea; National Research Foundation of Korea CBET-0932872 / National Science Foundation; National Science Foundation (NSF)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Civil, Architectural, and Environmental Engineering
- Web of Science ID
- WOS:000296756500022
- Scopus ID
- 2-s2.0-81055147918
- Other Identifier
- 991021931771904721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Engineering, Environmental
- Environmental Sciences