Journal article
Escherichia coli Inactivation by Water-Soluble, Ozonated C-60 Derivative: Kinetics and Mechanisms
Environmental science & technology, v 43(19), pp 7410-7415
01 Oct 2009
PMID: 19848154
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Antibacterial and bactericidal effects of a C-60 derivative prepared via excess ozonation of C-60 aggregates in water were investigated using Escherichia coli as a representative microorganism. Ozonated C-60 up to 10 mg/L did not cause significant growth inhibition nor inactivation of E coli in the absence of either oxygen or light. However, it readily inactivated E coli in the presence of both oxygen and light at much faster rate than parent C-60 aggregates, suggesting involvement of photochemical generation of reactive oxygen species (ROS). Among ROS, hydroxyl radical was found as a primary agent for the cell inactivation. Little surface protein release and concurrent intercellular enzyme degradation during the course of E coli inactivation, along with visual evidence obtained from transmission electron microscopic analysis, collectively indicated that the mechanism of E. coli inactivation by ozonated C-60 involves efficient penetration of ozonated C-60 into E coli and subsequent photochemical production of hydroxyl radical within the cell cytoplasm, These findings suggest that oxidative transformation of C-60 can lead to increased toxicity in addition to increased water solubility and reduced size, warranting further studies on other potential chemical derivatization possible in natural and engineered environments for an accurate assessment of environmental impact of this class of materials.
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Details
- Title
- Escherichia coli Inactivation by Water-Soluble, Ozonated C-60 Derivative: Kinetics and Mechanisms
- Creators
- Min Cho - Georgia Institute of TechnologyJohn D. Fortner - Georgia Institute of TechnologyJoseph B. Hughes - Rice UniversityJae-Hong Kim - Georgia Institute of Technology
- Publication Details
- Environmental science & technology, v 43(19), pp 7410-7415
- Publisher
- Amer Chemical Soc
- Number of pages
- 6
- Grant note
- D832526 / U.S. Environmental Protection Agency; United States Environmental Protection Agency
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Civil, Architectural, and Environmental Engineering
- Web of Science ID
- WOS:000270136500042
- Scopus ID
- 2-s2.0-70349612506
- Other Identifier
- 991021931901404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Engineering, Environmental
- Environmental Sciences